ANALYSIS OF ADHESIVE AND INVASION MECHANISM OF PIROPLASMA IN VECTOR TICK
| Project/Area Number |
15580265
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | KITASATO UNIVERSITY |
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Principal Investigator |
OYAMADA Takashi KITASATO UNIVERSITY, VETERINARY PARASITOLOGY, PROFESSOR, 獣医畜産学部, 教授 (80050665)
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| Co-Investigator(Kenkyū-buntansha) |
IKADAI Hiromi KITASATO UNIVERSITY, VETERINARY PARASITOLOGY, ASSISTANT PROFESSOR, 獣医畜産学部, 講師 (80327460)
YOSHIOKA Kazuki KITASATO UNIVERSITY, VETERINARY ANATOMY, ASSISTANT PROFESSOR, 獣医畜産学部, 講師 (30327457)
MATSUU Aya KITASATO UNIVERSITY, VETERINARY INTERNAL MEDICINE I, RESEARCH ASSOCIATE, 獣医畜産学部, 助手 (40348595)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | PIROPLASMA / HAEMAPHYSALIS LONGICORNIS / TRANSMISSOIN / BABESIA CABALLI / BABESIA EQUI / BABESIA GIBSONI / ADHESIVE / INVASION / モノクローナル抗体 / 伝播 / 付着および侵入機構 / Lactoferrin / ピロプラズマ原虫感染症 / 付着・侵入機構 / 遺伝子 / 組換え蛋白質 / Loop-mediated Isothermal Amplification Method / 迅速高感度簡易診断法 |
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| Research Abstract |
1.We investigated the usefulness of the needle injection method as an experimental means of transferring infection of equine Babesia parasites, namely Babesia caballi and Babesia equi, to the tick Haemapysalis longicornis. Further, we also determine investigated the transovarial and transstadial transmission of these parasites in this tick. Engorged adult or nymph ticks were injected with B.caballi- or B.equi-infected erythrocytes into the midgut from the integument by hypodermic needle. Detection of equine Babesia in ticks was by nested PCR for parasite DNA. For transovarial transmission, B.caballi and B.equi DNA were detected in adult ticks, laid eggs and larvae, respectively. For transstadial transmission, B. equi DNA was detected in adult ticks after molt of the injected nymph, and in salivary glands and carcass tissues of adult ticks after molt of the injected nymph. These findings support the potential role of H.longicornis in the transmission of equine Babesia.
2.We established mAbs against the Babesia surface protein and extracellular merozoite termed mAb, and used it to screen cDNA expression library prepared from Babesia merozoite mRNA for highly expressed proteins.
3.Real time-PCR was found to be reproducible and accurate for the quantification of parasite DNA in experimentally infection and far more sensitive than traditional microscopic examination. LAMP method provides a useful tool for the detecton of B.gibsoni infection in dog.
4.Apo-LF had the strongest inhibitory effect on B.caballi ; this may have been caused by inactivation or inhibition of a growth factor in the culture medium.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Inhibitory effect of lactoferrin on in vitro growth of Babesia cuballi.2005
Author(s)
Ikadai, H., Tanaka, T., Shibahara, N., Tanaka, H., Matsuu, A., Kudo, N., Shimazaki, K., Igarashi, I., Oyamada, T.
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Journal Title
Am.J.Trop.Med.Hyg. 73
Pages: 710-712
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Development of a SYBR Green real-time polymerase chain reaction assay for quantitative detection of Babesia gibsoni (Asian genotype) DNA.2005
Author(s)
Matsuu, A., Ono, S., Ikadai, H., Uchide, T., Imamura, S., Onuma, M., Okano, S., Higuchi, S.
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Journal Title
J.Vet.Diagn.Invest. 17
Pages: 569-573
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular characterization of a putative protein disulfide isomerase from Babesia caballi.2005
Author(s)
Ikadai, H., Tsukada, R., Sasaki, M., Takashiro, R., Yokoyama, N., Kudo, N., Igarashi, I., Oyamada, T.
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Journal Title
Parasitology 131
Pages: 775-782
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Molecular cloning and characterization of a putative binding protein of Babesia caballi.2005
Author(s)
Ikadai, H., Takamatsu, Y., Takashiro, R., Segawa, A., Kudo, N., Igarashi, I., Oyamada, T.
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Journal Title
Am.J.Trop.Med.Hyg. 73
Pages: 1135-1138
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method.2004
Author(s)
Ikadai, H., Tanaka, H., Shibahara, N., Matsuu, A., Uechi, M., Itoh, N., Oshiro, S., Kudo, N., Igarashi, I., Oyamada, T.
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Journal Title
J.Clin.Microbiol. 42
Pages: 2465-2469
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Incidence of canine Babesia gibsoni infection and subclinical infection among Tosa dog in Aomori Prefecture, Japan.2004
Author(s)
Matsuu, A., Kawabe, A., Koshida, Y., Ikadai, H., Okano, S., Higuchi, S.
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Journal Title
J.Vet.Med.Sci. 66
Pages: 893-897
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Efficacy of atovaquone against Babesia gibsoni in vivo and in vitro.2004
Author(s)
Matsuu, A., Koshida, Y., Kawahara, M., Inoue, K., Ikadai, H., Hikasa, Y., Okano, S., Higuchi, S.
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Journal Title
Vet.Parasitol. 124
Pages: 9-18
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ikadai, H., Tanaka H., Shibahara, N., Matsuu, A., Uechi, M., Itoh, N., Oshiro, S., Kudo, N., Igarashi, I., Oyamada, T.: "Molecular Evidence of Babesia gibsoni Parasite Infections in Japan and Evaluation of the Diagnostic Potential of a Loop-mediated Isothermal Amplification Method"Journal of Clinical Microbiology. (accepted)(掲載予定).
