A search by micro two-dimensional electrophoresis of an early marker in a diabetic nephropathy (2005)
| Project/Area Number |
15580280
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Azabu University |
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Principal Investigator |
SAKAGUTI Kazuko Azabu University, Environmental Health, associate professor, 環境保健学部, 助教授 (40153879)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Jun Azabu University, professor, 環境保健学部, 教授 (20075930)
SHIROTA Kinnji Azabu University, professor, 附属生物科学総合研究所, 教授 (70147974)
INOMATA Tomoo Azabu University, associate professor, 獣医学部, 助教授 (10147978)
KOJIMA Hisako Kitasato University School of Medicine, associate professor, 医学部, 助教授 (90118810)
NISHIYAMA Katuhiro Kanagawa Dental college, assistant professor, 講師 (20084783)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | diabetic nephropathy / early marker / two dimensional electrophoresis / タンパク質高感度染色 / ミクロ2次元電気泳動分析 / タンパク質高感度染色法 |
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| Research Abstract |
PURPOSE : We searched for early checkup marker of a diabetes kidney disease with a clinical animal as a model.
RESULT : Micro two-dimensional electrophoresis method analyzed a chronic kidney failure cat and plasma (1μL) of a diabetes cat in non-denaturation condition bottom ( I type) and non-denaturation-denaturation condition bottom (II type) and I used CBB dyeing and performed change analysis of a protein spot (the following, a spot). With chronic kidney failure cat plasma, IgM and two spots of Pre-albumin increased. Furthermore, increase of two spots was observed with II type in a low molecular weight domain. One this spot house which increased was identified with RBP by Western blot (WB) law. With diabetes cat plasma, increase was observed at one spot, and decrease was observed at two spots. The spot that increased was identified with Haptoglobin by WB method, but the spot that decreased is non-identification.
We used 10 times concentration urine (1μL) for 100 times concentration urine, renal insufficiency cat urine for diabetes cat urine and performed change analysis of a spot likewise. With diabetes and a protein image of renal insufficiency cat urine, I was able to observe decrease of hoptoglobin (Hp) and albumin (Alb), transferrin (TO, prealbumin (Pre-alb) and increase of a spot of beta2 microglobulin (β_2-M). Although it was observed, as for the renal insufficiency cat urine, as for the individual difference, increase of all spots was remarkable. On the other hand, as for the diabetes cat urine, as for Pre-alb, β_2-M, increase was slightly observed although Alb, increase of Tf were remarkable. As a result, in Alb and the spot of Tf, in Pre-alb and the spot of β_2-M, possibility as marker-of renal insufficiency was suggested for an index of diabetes.
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Report
(4 results)
Research Products
(11 results)
