Structure and function of synaptic vesicle transporter which supports glutamstergic chemical transduction.
| Project/Area Number |
15590060
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
OTUKA Masato Okayama university, Advanced Science Research Center, Research Associate, 自然生命科学研究支援センターゲノムプロテオーム解析部門, 助手 (30243489)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Glutamate / Membrane protein / Topology / トランスポーター |
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| Research Abstract |
Vesicular glutamate transporter (VGLUT) plays an essential role in L-glutamate signaling in neurons and some peripheral tissues through vesicular storage of L-glutamate in secretory vesicles. To investigate the topology of VGLUT in membranes, we prepared site-directed antibodies against the amino-terminal (anti-N), 1st putative loop (anti-L), and carboxyl terminal (anti-C) regions. None of the antibodies reacted with VGLUT2 expressed in COS cells because they could not gain access to the antigen. However, both the anti-N and anti-C antibodies recognized VGLUT2 when the cells were permeabilized with digitonin, while the anti-L antibodies did not. Immunological reactivity to anti-L-antibodies appeared when the cells were permeabilized with Triton X-100. These results suggest that both the amino-terminal and carboxyl-terminal regions of VGLUT2 in membranes face the cytoplasm while the 1st loop faces the lumen.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Vesicular storage and secretion of L-glutamate from glucagon-like peptide 1-secreting clonal intestinal L cells.2006
Author(s)
Uehara S, Jun S.K, Morimoto R, Arioka S, Miyaji T, Juge N, Hiasa M, Ishimura A, Otsuka M, Yamamoto A, Maechler P, Moriyama Y.
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Journal Title
J Neurochem. 96(2)
Pages: 550-560
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Co-expression of vesicular glutamate transporters (VGLUT1 and VGLUT2) and their association with synaptic-like microvesicles in rat pinealocytes.2003
Author(s)
Morimoto, R., Hayashi, M., Yatsushiro, S., Otsuka, M., Yamamoto, A., Moriyama, Y.
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Journal Title
J Neurochem. 84(2)
Pages: 382-391
Related Report
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[Publications] Morimoto, R, Hayashi, M., Yatsushiro, S., Otsuka, M., Yamamoto, A., Moriyama.Y.: "Co-expiession of vesicular glutamate transporters (VGLUT1 and VGLUT2) and their association with synaptic-like microvesides in rat pinealocytes"J Neurochem.. 84(2). 382-391 (2003)
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