Investigation of interstitial cells of Cajal and pacemaker mechanism of the gastrointestinal tract using new culture system
| Project/Area Number |
15590158
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nagoya University |
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Principal Investigator |
TORIHASHI Shigeko Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (90112961)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Shinsuke Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (30192230)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Pacemaker cell / Interstitial cells of Cajal / gastrointestinal tract / mouse ES cell / cell culture / development / BMP / 消化管運動 / ペースメーカー / 自動運動 / ES細胞 / PDGF / in situ hybridization / c-Kit / マウス胚性幹細胞(ES細胞) / 転写因子 |
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| Research Abstract |
In the period of 2003
We advanced the culture system of cell clusters isolated from the muscle layer of the mouse small intestine and confirmed that the cell cluster kept its original characteristic features in vitro. Using this method we isolated interstitial cells of Cajal(ICC) and immunize to rats to make a monoclonal antibody. We established the clone to produce antibody(AIC). This antibody is suitable for morphological studies because immunoreactivity was stable for paraformaldehyde fixation, and we applied for a patent. We plan to identify the epitope binding to AIC. Dr.Nakayama also advanced the study of pacemaker mechanism using this culture system.
In the period of 2004
The other culture system using mouse ES cells was studied and analyzed the formation of gut-like structures. We showed that formation process of gut-like structures mimicked the gut organogenesis, and several transcription factors crucial for the development of endoderm and gut organogenesis were expressed as a similar manner to mouse embryo. We plan to study differentiation of smooth muscles and ICC using this culture system.
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Report
(3 results)
Research Products
(38 results)
