| Project/Area Number |
15590168
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Iwate Medical University |
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Principal Investigator |
ISOGAI Sumio Iwate Medical University, School of Medicine, Department of Anatomy, Lecturer (60212966)
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| Project Period (FY) |
2003 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | Angiogenesis / Renal vascular system / Adrenal vascular system / Sea turtle / Micro dye injection / Micro resin injection / 後腎 / 腎臓 / 副腎 / 樹脂鋳型 / SEM |
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| Research Abstract |
Textbooks for embryology generally explain that the definitive renal (metanephric) artery derived from mesonephric arteries. We have verified the morphogenesis of pro-, meso- and meta-nephric vascular systems in salamander and rat embryos using our corrosive resin casting method. Our results suggested that the adrenal anlage, but not mesonephrose, plays key role in the renal vascular formation. This time we followed the morphogenetic process of pro-, meso- and meta-nephric vascular systems in a sea turtle using micro dye and resin injection methods.
In order to complete this study, we needed more than 500 well-injected specimens of sea turtle. We collected about 200 fertilized eggs under the permission from Yakushima-chou in each year during the term of this project (4 years). All the eggs were transported by a plane to Iwate medical university, and incubated at 30℃. We finally prepared a series of dye-injected specimens and a series of corrosive resin casts of sea turtle to accomplish this study.
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