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A Study on Central Regulatory mechanisms of Gasping using Genetically Engineered Mice

Research Project

Project/Area Number 15590181
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionAKITA UNIVERSITY

Principal Investigator

KATSUYA Yamada  Akita Univ., Sch.Med., Research Associate, 医学部, 助手 (40241666)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsgasping / pherenic nerve / medulla / hypoxia / ATP-sensitive potassium chennels / ATP-senstive potassium channnel / ATP-sensitive potassium channel
Research Abstract

When a prolonged, severe hypoxic condition is induced by ischemia or apnea, animals show a specific respiration pattern consisted of rapid and large inspiration with long respiration interval called gasping in order to maintain minimum oxygen supply and carbone dioxide removal. However, molecular mechanisms underlying central regulation of gasping is unclear.
In the present study, we found that mice lacking ATP-sensitive potassium (KATP) channels containing Kir6.2 subunit (KO mice) show minimum number of gasping after decapitation. Indeed, the number of gasps was significantly smaller, total duration of gasping, are shorter, and the onset of gasping is slower than those of wild-type mice. Similar results were also found in mice subjected to severe (〜5%) hypoxia under urethane-anesthesia. Recordings of phrenic nerve activity further revealed that KO mice did not show phrenice activity having waveform typical of normal gasping except for the last few gasps just before cessation of respiration, suggesting that KATP channels are critically involved in the induction of gasping during severe hypoxia. This hypothesis was further confirmed by mice restrained in a tube under unanesthetized conditions. The body movemement during gasping was successfully detected by piezoelectric device put beneath the mice and a CO2 monitor set within the tube.
On the other hand, Kir6.2-containing KATP channels are expressed most abundantly in GABAergic principal neurons in the midbrain nucleus substantia nigra pars reticulata (SNr), and exert important role for detecting severe hypoxic conditions (Yamada et al, J Mol Cell Cardiol, in press). Here we show that these neurons not only sense hypoxia but also mild hypoglycemia. In the present study, however, we found that the KATP channels in the SNr are not importantly involved in the detection of mild hypoglycemia (Yuan et al, Neurosci Lett, 2004).

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (15 results)

All 2005 2004 2003 Other

All Journal Article (12 results) Book (1 results) Publications (2 results)

  • [Journal Article] Neuroprotection by K_<ATP> channels.2005

    • Author(s)
      Yamada, K.
    • Journal Title

      J.Mol.Cell Cardiol. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of ABCA2 protein in human vestibular schwannoma and peripheral nerve2005

    • Author(s)
      Wang, Y.
    • Journal Title

      J.Neurol.Sci. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neuroprotection by K_<ATP> channels.2005

    • Author(s)
      Yamada, K.
    • Journal Title

      J.Mol.Cell Cardiol. in press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of ABCA2 protein in human vestibular schwannoma and peripheral nerve2005

    • Author(s)
      Wang, Y.
    • Journal Title

      J.Neurol.Sci. in press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mulitiminute oscillations in mouse subsutantia nigra pars reticulata neurons in vitro.2004

    • Author(s)
      Yuan, H.
    • Journal Title

      Neurosci.Lett 355

      Pages: 136-140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Glucose sensitivity in mouse substantia nigra pars reticulata neurons in vitro.2004

    • Author(s)
      Yuan, H.
    • Journal Title

      Neurosci.Lett 355

      Pages: 173-176

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Cloning, tissue distribution and function of ABCA transporters.2004

    • Author(s)
      Inagaki, N.
    • Journal Title

      International Congress Series 1262

      Pages: 578-581

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Glucose sensitivity in mouse subsutantia nigra pars reticulata neurons in vitro.2004

    • Author(s)
      Yuan, H.
    • Journal Title

      Neurosci.Lett 355

      Pages: 173-176

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular and functional diversity of ATP-sensitive K+ channels : the pathophysiological roles and potential drug targets.2003

    • Author(s)
      Nakaya H.
    • Journal Title

      Folia Pharmacol JPN 122

      Pages: 243-250

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 各種臓器のATP感受性K^+チャネルの構造と機能:薬物制御におけるQOLの向上を目指して2003

    • Author(s)
      中谷, 他
    • Journal Title

      日薬理誌 122

      Pages: 249-250

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Neuroprotection by KATP channels.

    • Author(s)
      Yamada, K.
    • Journal Title

      J.Mol.Cell.Cardiol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of ABCA2 protein in human vestibular schwannoma and peripheral nerve.

    • Author(s)
      Wang, Y.
    • Journal Title

      J.Neurol.Sci. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 日薬理誌2003

    • Author(s)
      中谷, 他
    • Total Pages
      271
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Yuan, H.: "Multiminute oscillations in mouse substantia nigra pars reticulata neurons in vitro"Neurosci.Lett.. 355. 136-140 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yuan, H.: "Glucose sensitivity in mouse substantia nigra pars reticulata neurons in vitro"Neurosci.Lett.. 355. 173-176 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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