| Project/Area Number |
15590190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Keio University |
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Principal Investigator |
OKADA Yasumasa Keio University, Department of Medicine, Associate Professor, 医学部, 助教授 (80160688)
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| Co-Investigator(Kenkyū-buntansha) |
KUWANA Shun-ichi Teikyo University, Department of Medicine, Assistant Professor, 医学部, 講師 (70129998)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | respiratory control / central chemosensitivity / propofol / brainstem / respiratory neuronal network / voltage imaging / GABA / muscle relaxant / 中枢化学受容 / 延髄 / ニューロン / アセチルコリン / コリンエステラーゼ / カリウムチャネル / CO_2リセプター / 橋 / 高濃度CO_2液微量注入 / 延髄腹側表面部 / ニコチン性アセチルコリン受容体 / 神経回路網機 / 延髄腹側 / 膜電位感受性色素 / 神経活動イメージング / 電気刺激 |
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| Research Abstract |
In this research project we have studied the function and anatomy of the neural substrate for the central respiratory chemoreception and central respiratory control.
In order to identify the functional role of an important neurotransmitter GABA in central respiratory control, we used GABA-synthesizing enzyme (GAD) gene-targeted mice, and found that GABA is necessary in the maintenance of stable respiratory output (Kuwana et al. 2003 ; 2004 ; 2006). We also found that respiratory depression by propofol is induced by its GABA-like inhibitory action (Kashiwagi et al. 2004 ; 2004).
We applied a voltage imaging technique to an in vitro brainstem preparation, and succeeded functional mapping of chemosensitive regions. We reported that the pontine A5 region has intrinsic chemosensitivity (Ito et al. 2004).
On the basis of our hypothesis that nicotinic acetylcholine receptor is importantly involved in central respiratory chemoreception, we analyzed the effects of non-depolarizing muscle relaxants on chemoresponsiveness in vitro. We first reported that non-depolarizing muscle relaxants inhibit respiratory activity in the brainstem (Sakuraba et al. 2003 ; 2005).
We summarized the results of our studies, and have proposed our theories that potassium channels play a key role as the chemosensor in chemoreceptor cells (Oyamada et al. 2006) and that specific small cells surrounding fine vessels in the superficial ventral medulla are primary chemoreceptor cells (Okada et al. 2006).
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