The Role of proteoglycans in chondrocyte differentiation of mesenchymal stem cells
| Project/Area Number |
15590355
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Aichi Medical University |
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Principal Investigator |
WATANABE Hideto Aichi Medical University, Institute for Molecular Science of Medicine, Associate Professor, 分子医科学研究所, 助教授 (90240514)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | cartilage / chondrocyte differentiation / mesenchymal stem cell / TGF-beta / proteoglycnn / versican / PG-M / ultrasound / BMP / TGF-β |
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| Research Abstract |
1. We evaluated the effects of ultrasound (US) on differentiation of mesenchymal stem cells (MSCs) toward chondrocyte snarl cartilage matrix formation. When human MSCs cultured in the pellets were treated with transforming growth factor-beta (TGF-b), they differentiated into chondrocytes as assessed by alcian blue staining and immunostaining for aggrecan, but non-treated cell pellets and cell pellets treated with BMP-6 alone did not. Furthermore, when low-intensity US was applied for 20 min everyday to the TGF-beta-treated cell pellets, chondrocyte differentiation was enhanced. These results suggest that TGF-b is essential for chondrocyte differentiation of MSCs in pellets and US enhances the differentiation.
2. Using chondrogenic/chondrocytic N1511 cells, we evaluated the role of versican/PG-M in chondrocyet differentiation. N1511 cells exhibited transient high expression of versican at an early stage and that of aggrecan at a late stage, which reflects their in vivo expression patterns during chondrocyte differentiation. When versican expression was inhibited using an antisense technology, the following aggrecan expression was decreased and delayed, as assessed by realtime RT-PCR. These results suggest that transient versican expression at the early stage positively regulate chondrocyte differentiaion.
3. In addition to high transient expression, versican is continuously expressed, especially in the articular cartilage. We found that versican is colocalized with both hyaluronan and link protein there. Biochemical analysis further confirmed the presence of versican aggregate. These results suggest the presence of tow proteoglycan aggregates, which may play distinct roles.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Heparan sulfate of perlecan is involved in glomerular filtration.2005
Author(s)
Morita, H., Yoshimura, A., Inui, K., Ideura, T., Watanabe, H., Wang, L., Soininen, R., Tryggvason, K.
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Journal Title
J.Am.Soc.Nephrol. 16-6
Pages: 1703-1710
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Ultrasound enhances chondrocyte differentiation of human mesenchymal stem cells.2004
Author(s)
Ebisawa, K., Hata, K., Okada, K., Kimata, K., Ueda, M., Torii, S., Watanabe, H.
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Journal Title
Tissue Engineering 10-5,6
Pages: 921-929
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Distinct interaction of versican/PG-M with hyaluronan and link protein.2003
Author(s)
Matsumoto, K., Shionyu, M., Go, M., Shimizu, K., Shinomura, T., Kimata, K., Watanabe, H.
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Journal Title
J.Biol.Chem. 278-42
Pages: 41205-41212
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Proteoglycan aggregate of versican/PG-M in cartilage.
Author(s)
Matsumoto, K., Kamiya, N., Atsumi, F., Shimizu, K., Shinomura, T., Yamada, Y., Kimata, K., Watanabe, H.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
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