Development of effective mucosal vaccine against bacterial afferent infectious diseases
| Project/Area Number |
15590395
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bacteriology (including Mycology)
|
|---|
| Research Institution | NAGOYA CITY UNIVERSITY |
|---|
Principal Investigator |
TANIGUCHI Toru Nagoya City University, Graduate School of Medical Sciences, Assistant Professor, 大学院・医学研究科, 講師 (00285199)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ISAKA Masanori Nagoya City University, Graduate School of Medical Sciences, Research Associate, 大学院・医学研究科, 助手 (40336673)
IIDA Tetsuya Osaka University, Research Institute for Microbial Diseases, 微生物病研究所, 助教授 (90221746)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | enterotoxigenic Escherichia coli / Vibrio parahaemolyticus / pili / colonization factor / adhesin / thermostable direct hemolvsin / mucosal vaccine / immune response |
|---|
| Research Abstract |
For prevention (vaccine) of enterotoxigenic Escherichia coli (ETEC) and Vibrio parahaemolyticus infections, it is important to stimulate the production of mucosal secretory immunoglobulin A (sIgA) for blocking of colonization to intestinal epithelium and neutralizing of toxins. Recombinant cholera toxin B subunit (rCTB) was used as a mucosal adjuvant.
1. Purification of vaccine antigen
Adhesins (CfaE and CofJ) of pilus colonization factor antigens (CFA/I and CFA/III) of ETEC and less hemolytic mutant toxin of thermostable direct hemolysin (mTDH) of V. parahaemolyticus were purified by using His-tag fusion vector (pQE-30Xa).
2. Immune response in experiment animal
Intranasal administration of CfaE or CofJ to BALB/c mice with and without rCTB induced CfaE-and CofJ-specific serum IgG and mucosal immune response (sIgA) in the saliva, the nasal cavity, the lung, the vagina, the small intestine and the feces.
BALB/c mice immunized intranasally with mTDH irrespective of the presence of rCTB showed TDH-specific serum IgG antibody response and mucosal immune response (sIgA), especially in the lung and the small intestine. Moreover, mice immunized could protect against lethal challenge with wild-type TDH.
|
Report
(3 results)
Research Products
(12 results)
