the role of adaptor protein SH2-B in adipocyte differentiation

• Project/Area Number: 15590522
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Ritsumeikan University
• Principal Investigator: SUZUKI Kenji Ritsumeikan University, Department of Information Science and Engineering, Professor, 情報理工学部, 教授 (10187726)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
• Keywords: adipocyte / tyrosine kinase / adaptor protein / signal transduction

Research Abstract

Expression of SH2-B splicing variants in 3T3-L1 cells during adipocyte differentiation.
Preadipocyte cell line mouse 3T3-L1 is extensively used to investigate the molecular mechanisms in adipogenesis. To evaluate adipocyte differentiation, three different methods such as Oil red-O staining, RT-PCR for adipocyte specific genes and GPDH assay, were used. Among 4 isoforms of SH2-B, expression of β- andγ- isoforms were detected in 3T3-L1 cells, and these expression levels were up-regulated during adipogenesis. Expression of SH2-B family proteins was also investigated. Changes in expression level of APS was similar to these of SH2-B splicing variants, although mRNA level of lnk did not change during adipogenesis.
Effect of overexpression or suppression of SH2-B in 3T3-L1 cells on adipocyte differentiation.
Willd-type and mutated human SH2-Bβ were introduced into 3T3-L1 cells by using a lipofection method. The expression of human SH2-B was confirmed by RT-PCR and western blot analysis. The expression of wild-type SH2-B accelerated the cellular differentiation into adipocytes. On the other hand, the mutated SH2-B, which lacks the ability to bind to phosphorylated proteins, suppressed its differentiation. The expression of small interfering RNA against SH2-B also inhibited adipogenesis in 3T3-L1 cells.
In conclusion, these results clearly indicate that SH2-B plays a pivotal role in adipocyte differentiation.

Research Products

• [Journal Article] Genetic variation in hypoxia-inducible factor 1-alpha and its possible association with high altitude adaptation in Sherpas. (2003)
  • Author(s): Suzuki, K. et al.
  • Journal Title: Med.Hypotheses 61(3) Pages: 385-389
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Gliclazide protects pancreatic beta-cells from damage by hydrogen peroxide. (2003)
  • Author(s): Kimoto, K. et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 303(1) Pages: 112-119
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effects of Nutrient and Food Intake on Calcaneous Bone Mass among Healthy Japanese Women in the Predelivery and Postpartum Periods (2003)
  • Author(s): Sekine, Y. et al.
  • Journal Title: J.Women.Health 12(7) Pages: 643-653
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Intermittent Hypobaric Hypoxia Increases the Ability of Neutrophils to Generate Superoxide Anion in Humans. (2003)
  • Author(s): Hitomi, Y. et al.
  • Journal Title: Clin.Exp.Pharmacol.Physiol. 30(9) Pages: 659-664
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Physical Exercise and Extracellulrar Superoxide ismutase : Antiatherogenic Effects. (2003)
  • Author(s): Ohno, H. et al.
  • Journal Title: Adv.Exerc.Sports Physiol. 9(2) Pages: 45-53
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effect of Eccentric Exercise on Neutrophil Function in Human Plasma. (2003)
  • Author(s): Miyazaki, H. et al.
  • Journal Title: Adv.Exerc.Sports Physiol. 9(2) Pages: 69-75
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Genetic variation in hypoxia-inducible factor 1-alpha and its possible association with high altitude adaptation in Sherpas. (2003)
  • Author(s): Suzuki, K., Kizaki, T., Hitomi, Y., Nukita, M., Kimoto, K., Miyazawa, N., Kobayashi, K., Ohnuki, Y., Ohno, H.
  • Journal Title: Med.Hypotheses 61(3) Pages: 385-389
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gliclazide protects pancreatic beta-cells from damage by hydrogen peroxide. (2003)
  • Author(s): Kimoto, K., Suzuki, K., Kizaki, T., Hitomi, Y., Ishida, H., Katsuta, H., Itoh, E., Ookawara, T., Suzuki, K., Honke, K., Ohno, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 303 Pages: 112-119
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of Nutrient and Food Intake on Calcaneous Bone Mass among Healthy Japanese Women in the Predelivery and Postpartum Periods. (2003)
  • Author(s): Sekine, Y., Matsunaga, N., Kokaze, A., Yoshida, M., Suzuki, K., Ohno, H., Takashima, Y.
  • Journal Title: J.Women Health 12(7) Pages: 643-653
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Intermittent Hypobaric Hypoxia Increases the Ability of Neutrophils to Generate Superoxide Anion in Humans. (2003)
  • Author(s): Hitomi, Y, Miyamura, M., Mori, S., Suzuki, K., Kizaki, T., Itoh, C., Murakami, K., Haga, S., Ohno, H.
  • Journal Title: Clin.Exp.Pharmacol.Physiol. 30(9) Pages: 659-664
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Physical Exercise and Extracellulrar Superoxide Dismutase : Antiatherogenic Effects. (2003)
  • Author(s): Ohno, H., Kizaki, T., Suzuki, K., Hitomi, Y., Watanabe, S., Ookawara, T., Suzuki, K., Haga, S., Saitoh, D., Radak, Z., Ji, L.L.
  • Journal Title: Adv.Exerc.Sports Physiol. 9(2) Pages: 45-53
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of Eccentric Exercise on Neutrophil Function in Human Plasma. (2003)
  • Author(s): Miyazaki, H., Haga, S., Suzuki, K., Toshinai, K., Esaki, K., Oh-ishi, S., Hitomi, Y., Kizaki, T., Ji, L.L., Ohno, H.
  • Journal Title: Adv.Exerc.Sports Physiol. 9(2) Pages: 69-75
  • NAID: 110002706335
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Is Angiotensin I-Converting Enzyme I/D Polymorphism Associated with Endurance Performance and/or High Altitude Adaptation ?
  • Author(s): Ohno, H.et al.
  • Journal Title: Adv.Exerc.Sports Physiol. (印刷中)(in press)
• [Publications] Suzuki, K. et al.: "Genetic variation in hypoxia-inducible factor 1-alpha and its possible association with high altitude adaptation in Sherpas."Med.Hypotheses. 61(3). 385-389 (2003)
• [Publications] Kimoto, K. et al.: "Gliciazide protects pancreatic beta-cells from damage by hydrogen peroxide."Biochem.Biophys.Res.Commun.. 303(1). 112-119 (2003)
• [Publications] Sekine, Y. et al.: "Effects of Nutrient and Food Intake on Calcaneous Bone Mass among Healthy Japanese Women in the Predelivery and Postpartum Periods"J.Women Health. 12(7). 643-653 (2003)
• [Publications] Hitomi, Y. et al.: "Intermittent Hypobaric Hypoxia Increases the Ability of Neutrophils to Generate Superoxide Anion in Humans."Clin.Exp.Pharmacol.Physiol.. 30(9). 659-664 (2003)
• [Publications] Ohno, H. et al.: "Physical Exercise and Extracellulrar Superoxide ismutase : Antiatherogenic Effects."Adv.Exerc.Sports Physiol.. 9(2). 45-53 (2003)
• [Publications] Miyazaki, H. et al.: "Effect of Eccentric Exercise on Neutrophil Function in Human Plasma."Adv.Exerc.Sports Physiol.. 9(2). 69-75 (2003)