| Project/Area Number |
15590532
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
OHYAMA Masayuki Osaka Prefectural Institute of Public Health, DEPARTMENT OF ENVIRONMENTAL HEALTH, SENIOR RESEARCHER, 生活環境部, 主任研究員 (40175253)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | diesel exhaust particles / suspendedn particulate matter / macrophage / reactive oxygen species / superoxide / hydroxyl radical / 3-nirobenzanthrone / fine particle / スーパーオキサイド / 3-ニトロベンズアントロン / 微粒子 / macrophage / superoxide / man-made mineral fibers / sillca |
|---|
| Research Abstract |
Oxidative stress has emerged as a pivotal mechanism that underlies the toxic pulmonary effects of Suspended Particulate Matter (SPM). Experimental evidence shows that redox-active transition metals, redox-cycling quinoids and polycyclic aromatic hydrocarbons (PAHs) contained in SPM act synergistically, producing reactive oxygen species (ROS). The direct production of superoxide anion and the damaging hydroxyl radical has been studied in aqueous and DMSO suspensions of SPM both with and without H_2O_2, however, no study has reported on the release of ROS from ingesting macrophages with SPM. We investigated the time course of the ability to induce lucigenin-dependent Chemiluminescence (CL) from human monocyte-derived macrophages exposed to SPM, carbon black particles, and diesel exhaust particles (DEP). We also examined Hydroxyl radical generation from the same experimental system using the 2-deoxy-d-robse method. We found an increase of CL for SPM, but not for carbon black particles or for DEP Hydroxyl radical generation was observed in both SPM and DEP, but the release from DEP was more frequent than that from SPM. These results suggest that certain components of SPM are important in the response of ROS from ingesting macrophages with SPM, and that those components are dispersed from SPM into the atmosphere.
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