Transcriptional regulation of the human ABO histo-blood group genes is dependent on the negative regulation through the N box upstream of the proximal promoter
| Project/Area Number |
15590575
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Gunma University |
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Principal Investigator |
KOMINATO Yoshihiko Gunma University, Department of Legal Medicine and Molecular Genetics, Associate Professor, 大学院・医学系研究科, 助教授 (30205512)
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| Co-Investigator(Kenkyū-buntansha) |
HATA Yukiko Toyama Medical & Pharmaceutical University, Department of Legal Medicine, Researcher, 医学部, 教務職員 (30311674)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | ABO gene / transcription / N box / repression / promoter / ABO遺伝子 |
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| Research Abstract |
Our previous studies of the transcriptional regulatory mechanism of the human ABO gene indicated that negative regulatory elements are present in the sequence upstream of the proximal promoter, suggesting that transcription from the ABO proximal promoter is in part controlled by silencer elements just upstream of the promoter. In this study, we have confirmed that ABO gene expression is negatively regulated by the sequence upstream of the proximal promoter in KATOIII cells, and that the region between -202 and -118 is involved in the negative regulation in cells of both epithelial and erythroid lineages. Transient transfection experiments in KATOIII cells using a luciferase reporter plasmid carrying mutated N box at -196 to -191 demonstrated that the N box is a negative regulatory element in the -202 to -118 sequence. Using electrophoretic mobility shift assay, we showed that the N box binds with a nuclear factor, termed RACP, derived from KATOIII cells. These results suggest that repression of transcription from the ABO proximal promoter is partially dependent upon the N box, and that down-regulation of RACP may relieve the repression, thereby leading to expression of the ABO gene during maturation of cells in the epithelial lineage as well as the erythroid lineage.
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Report
(3 results)
Research Products
(22 results)
