Development of complementary and alternative medicine against antibiotic-resistant Helicobacter pylori infection
| Project/Area Number |
15590602
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
KITA Masakazu Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Associate Professor, 医学研究科, 助教授 (60153087)
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| Co-Investigator(Kenkyū-buntansha) |
IMANISHI Jiro Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Professor, 教授 (40112510)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Helicobacter / antibiotic-resistant / herbal medicine / essential oil / complementary medicine / alternative medicine / Helicobacter pylori / 生薬 / 補完・代替療法 |
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| Research Abstract |
Helicobacter pylori (H.pylori) is now recognized as an important causal agent in gastroduodenal diseases, such as chronic gastritis, peptic ulceration, gastric adenocarcinoma and mucosa-associated lymphoid tissue lymphoma (MALToma). Although the eradication of H.pylori using antibiotics often improves gastroduodenal diseases, resistance to the antibiotics is emerging. In this study, the antimicrobial effects of essential oils and herbal medicines were evaluated in vitro and in vivo. Thirteen essential oils used in this study completely inhibited the growth of H.pylori in vitro at a concentration of 0.1% (vol/vol). Cymbopogon citratus (lemongrass) and Lippia citriodora (lemon verbena) were bactericidal against H.pylori at 0.01% at pH 4.0 and 5.0. Resistance to lemongrass did not develop even after 10 sequential passages, whereas resistance to clarithromycin developed under the same conditions. In in vivo studies, the density of H.pylori in the stomach of mice treated with lemongrass was significantly reduced compared with untreated mice. On the other hand, the antibacterial effects of 18 herbal medicines were also evaluated in vitro and in vivo. Rhei Rhizoma, Artemisiae Capillari Flos and Caryophylli Flos inhibited the growth of H.pylori at a dose of 1mg/ml or more in vitro. Furthermore, the density of H.pylori in the stomach of mice treated with these herbal medicines was significantly reduced. These results demonstrate that the essential oils and herbal medicines are bactericidal against H.pylori without the development of acquired resistance, suggesting that essential oils and herbal medicines may have potential as complementary and alternative medicines for treatment of antibiotic-resistant H.pylori infection.
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Report
(4 results)
Research Products
(8 results)
