Anti-tumor effectof 15d-PGJ2 on human hepatoma cells
| Project/Area Number |
15590660
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAKAO Kazuhiko Nagasaki University, Health Research Center, Associate Professor, 保健管理センター, 助教授 (00264218)
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| Co-Investigator(Kenkyū-buntansha) |
ISHII Nobuko Nagasaki University, Health Research Center, Professor, 保健管理センター, 教授 (20088868)
HAMASAKI Keisuke Nagasaki University Hospital, Lecturer, 医学部・歯学部附属病院, 講師 (50333521)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | 15d-PGJ2 / TRAIL / TNF-α / hepatoma / NF-κB / STAT3 |
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| Research Abstract |
15d-PGJ2,a natural ligand of PPAR-γ, plays a key role in the differentiation of adipocytes and inhibits the cytokine-mediated NF-κB activation in various kinds of cells. Recently, it has been reported that 15d-PGJ2 has an anti-tumor activity in certain cancer cells. Therefore, in the present study, we evaluated the anti-tumor effect of 15d-PGJ2 on human hepatoma cells. 15d-PGJ2 inhibited the growth of HuH-7 human hepatoma cells in a dose-dependent manner. The cell cycle analysis revealed that 15d-PGJ2 induced G1 cell cycle arrest and small amounts of apoptosis in HuH-7 cells. Western blotting showed that 15d-PGJ2 down regulated the expression of cyclin D1,c-myc, Bcl-xL and survivin which are key molecules of growth and survival of cells, and inhibited the constitutive activation of STAT-3 in HuH-7 cells. In addition, pre-treatment of HuH-7 cells with 15d-PGJ2 greatly enhanced the Tumor necrosis factor(TNF)-related apoptosis-inducing ligand(TRAIL)-mediated apoptosis of these cells, in which TRAIL-induced NF-κB activation was dramatically repressed. These results suggest that 15d-PGJ2 has an anti-tumor activity against hepatoma cells through repressing both survival and growth signals of these cells
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Interferon-α sensitizes human hepatoma cells to TRAIL-induced apoptosis through DR5 upregulation and NF-κB inactivation.2003
Author(s)
Shigeno M, Nakao K, Ichikawa T, Suzuki K, Kawakami A, Abiru S, Miyazoe M, Nakagawa Y, Ishikawa H, Hamasaki K, Nakata K, Ishii N, Eguchi K.
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Journal Title
Oncogene 22(11)
Pages: 1653-1662
Description
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