A novel therapy for gastrointestinal cancer with calreticulin/CEA DNA vaccine
| Project/Area Number |
15590665
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Sapporo Medical University |
|---|
Principal Investigator |
OKAMOTO Tetsuro Sapporo Medical University, School of Medicine, the 4^<th> Department of Internal Medicine, Instructor, 医学部, 助手 (60332912)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥2,900,000 (Direct Cost: ¥2,900,000)
|
|---|
| Keywords | DNA vaccine / Calreticulin / CEA / immunotherapy |
|---|
| Research Abstract |
Carcinoembryonic antigen (CEA) has been identified as an attractive target for active vaccination approaches against gastrointestinal. Several clinical studies using DNA vaccine encoding CEA have been assessed. However its safety and immumogenicity have been established, clinical response stayed in only their stabilization of diseases. Expecting more effective immune responses, clinical trials were planned to combine with several adjuvants or cytokines, but they did not show additional responses. Calreticulin (CRT) can enhance antigen specific T cell mediated immune responses and have antiangiogenic effect by the function of its Ca2+binding protein. Therefore we engineered an innovative DNA vaccine, which expressing CRT chimerically liked to CEA (pcCRT/CEA) for the treatment of mice model bearing CEA expressing tumor, we also generated no insert (pcDNA3.1), CRT (pcCRT) and CEA (pcCEA) expressing plasmids. DNA vaccination was performed twice (day 0 and 7) intramuscularly and on day 21 tumor cells were inoculated subcutaneously. The tumor size on the mice immunized with pcCEA was smaller than with pcDNA3.1 or with pcCRT alone. And the group vaccinated mixed vaccine (pcCRT and pcCEA) generated smallest tumors, furthermore the mice group received pcCRT/CEA vaccination remained tumor free for 25 days after inoculation of the tumor cells. Thus DNA vaccine therapy using CRT linked to CEA holds promise for treating gastrointestinal cancers.
|
Report
(3 results)
Research Products
(10 results)
-
-
-
-
[Journal Article] Glutathione-S-transferase P1-1 protects aberrant crypt foci from apoptosis induced by deoxycholic acid2004
Author(s)
Nobuoka A, Takayama T, Miyanishi K, Sato T, Takanashi K, Hayashi T, Kukitsu T, Sato Y, Takahasni M, Okamoto T, Matsunaga T, Kato J, Oda M, Azuma T, Niitsu Y.
-
Journal Title
Gastroenterology. 127(2)
Pages: 428-43
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Successful, treatment with prostaglandin E1 of ischemic colitis complicated by colonic stricture2004
Author(s)
Abe S, Takayama T, Ohta H, Takimoto R, Okamoto T, Sato Y, Nikaido T, Takizawa K, Abe T, Niitsu Y.
-
Journal Title
Gastrointest Endosc. 60(1)
Pages: 148-51
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Fas-mediated apoptosome formation is dependent on reactive oxygen species derived from mitochondrial permeability transition in Jurkat cells2004
Author(s)
Sato T, Machida T, Takahashi S, Iyama S, Sato Y, Kuribayashi K, Takada K, Oku T, Kawano Y, Okamoto T, Takimoto R, Matsunaga T, Takayama T, Takahashi M, Kato J, Niitsu Y.
-
Journal Title
J Immunol. 173(1)
Pages: 285-96
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
[Journal Article] Allogeneic bone marrow transplantation in a patient with T prolymphocytic leukemia with small-intestinal involvement2003
Author(s)
Murase K, Matsunaga T, Sato T, Kuribayashi K, Kogawa K, Kawano Y, Okamoto T, Takayama T, Watanabe H, Niitsu Y, Hirayama Y.
-
Journal Title
Int J Clin Oncol. 8(6)
Pages: 391-4
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Argon plasma coagulation for successful treatment of early gastric cancer with intramucosal invasion2003
Author(s)
Sagawa T, Takayama T, Oku T, Hayashi T, Ota H, Okamoto T, Muramatsu H, Katsuki S, Sato Y, Kato J, Niitsu Y.
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
