Development of anti-tuberculosis vaccine using cytokine gene-transduced dendritic cells

• Project/Area Number: 15590803
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: SUDA Takafumi Hamamatsu University School of Medicine, 2^<nd> Division of Internal Medicine, Assistant Professor, 医学部, 助手 (30291397)
• Co-Investigator(Kenkyū-buntansha): CHIDA Kingo Hamamatsu University School of Medicine, 2^<nd> Division of Internal Medicine, Associate Professor, 医学部, 助教授 (40197611) ; NAGATA Toshi Hamamatsu University School of Medicine, Department of microbiology and Immunology, Associate Professor, 医学部, 助教授 (90275024)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,300,000); Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000); Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: Dendritic cell / vaccine / tuberculosis

Research Abstract

In the present study, we evaluated antigen 85A (Ag85A)-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A and injected to BALB/c mice intravenously. The DCs vaccine was capable of generating purified protein derivative (PPD)-, and the antigen-specific spleen cell proliferation and IFN-gamma production from the spleen cells. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) exhibiting lytic activity and IFN-gamma production specific to a CTL epitope on Ag85A molecule. This eliciting cellular immunity led to induction of moderate protective immunity against Mycobacterium tuberculosis

Research Products

• [Journal Article] Impaired TLR9 expression in alveolar macrophages with no sensitivity to CpG (2005)
  • Author(s): Suzuki K, Suda T, et al.
  • Journal Title: Am J Respir Crit Care Med (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differences in clinical features and prognosis of interstitial pneumonia between Polymyositis and dermatomyositis (2005)
  • Author(s): Fujisawa T, Suda T, et al.
  • Journal Title: J Rheumatol 32 Pages: 58-64
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differences in clinical features and prognosis of interstitial lung diseases between polymyositis and dermatomyositis. (2005)
  • Author(s): Fujisawa T, Suda T, Nakamura Y, Enomoto N, Ide K, Toyoshima M, Uchiyarna H, Tarnura R, Ida M, Yagi T, Yasuda K, Genma H, Hayakawa H, Chida K, Nakamura H
  • Journal Title: J Rheumatol 32(1) Pages: 58-64
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Occupational and Environmental Factors and Idiopathic Pulmonary Fibrosis in Japan. (2005)
  • Author(s): Miyake Y, Sasaki S, Yokoyama T, Chida K, Azuma A, Suda T, Kudoh S, Sakamoto N, Okamoto K, Kobashi G, Washio M, Inaba Y, Tanaka H.
  • Journal Title: Ann Occup Hyg (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Impaired Toll-like Receptor 9 Expression in Alveolar Macrophages with no Sensitivity to CpG DNA. (2005)
  • Author(s): Suzuki K, Suda T, Naito T, Ide K, Chida K, Nakamura H.
  • Journal Title: Am J Respir Crit Care Med (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Alpha-galactosylceramide, a ligand of natural killer T cells, inhibits allergic airway inflammation. (2005)
  • Author(s): Matsuda H, Suda T, Sato J, Nagata T, Koide Y, Chida K, Nakamura H.
  • Journal Title: Am J Respir Cell Mol Biol (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The incidence and clinical charactaristics of bronchiolitis obliterans organizing pneumonia syndrome after radiation therapy for breast cancer (2004)
  • Author(s): Miwa S, Suds T, et al.
  • Journal Title: Sarcoidosis Vasc Diffuse Lung Dis 21 Pages: 212-218
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The incidence and clinical charactaristics of bronchiolitis obliterans organizing pneumonia syndrome after radiation therapy for brest cancer. (2004)
  • Author(s): Miwa S, Morita S, Suda T, Suzuki K, Hayakawa H, Chida K, Nakamura H
  • Journal Title: Sarcoidosis Vase Diffuse Lung Dis 21(3) Pages: 212-218
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Vegetable, Fruit, and cereal intake and risk of idiopathic pulmonary fibrosis in Japan. (2004)
  • Author(s): Miyake Y, Sasaki S, Yokoyama T, Chida K, Azuma A, Suda T, Kudoh S, Sakamoto N, Okarnoto K, Kobayashi G, Washio M, Inaba Y, Tanaka H
  • Journal Title: Ann Nutr Metab 48(6) Pages: 390-397
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The incidence and clinical charactaristics of bronchiolitis obliterans organizing pneumonia syndrome after radiation therapy for breast cancer (2004)
  • Author(s): Miwa S, Suda T, et al.
  • Journal Title: Sarcoidosis Vasc Diffuse Lung Dis 21 Pages: 212-218
• [Journal Article] Impaired TLR9 expression in alveolar macrophages with no sensitivity to CpG
  • Author(s): Suzuki K, Suda T, et al.
  • Journal Title: Am J Respir Crit Care Med In press
• [Publications] Suda T, et al.: "High-dose intravenous glucocorticoid therapy abrogates circulating dendritic cells"J Allergy Clin Immunol. 112. 1237-1239 (2003)
• [Publications] Asada K, et al.: "Antiinflammatory roles of peroxisome proliferator-activated receptor gamma in human alveolar macrophages"Am J Respir Crit Care Med. 169. 195-200 (2004)
• [Publications] Yokomura K, et al.: "Increased expression of the 25-hydroxyvitamin D(3)-lalpha-hydroxylase gene in alveolar macrophages of patients with lung cancer"J Clin Endocrinol Metab. 88. 5704-5709 (2003)
• [Publications] Nakamura Y, et al.: "Induction of protective immunity to Listeria monocytogenes with dendritic cells retrovirally transduced with a cytotoxic T lymphocyte epitope minigene"Infect Immun. 71. 1748-1754 (2003)