Evaluation of exhaled breath condensate as a clinical tool for diagnosis and managements of patients with lung cancer

• Project/Area Number: 15590806
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Kyoto University
• Principal Investigator: MIO Tadashi Kyoto University, Respiratory Medicine, Lecturer, 医学研究科, 講師 (90243097)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Hisako Kyoto University, Respiratory Medicine, Assistant Professor, 医学研究科, 助手 (60359809)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥2,700,000 (Direct Cost: ¥2,700,000); Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: Exhaled breath condensate / lung cancer / tumor marker / cytokine / prostaglandin

Research Abstract

Recently, many studies were performed to
There has recently been exhaled breath condensate is an non-invasive method to
The breath condensates can be collected easily from patients without any invasion, and repeated collection from the same patients is possible. These features seem to fit for screening test or for management of patients. The aim of this study is to evaluate clinical utility in diagnosis and management of patients with lung cancer. Breath condensates were collected from 23 patients with lung cancer and 10 healthy volunteers. We tried to measure tumor makers, inflammatory and fibrotic cytokines and prostanoids. As for tumor markers, we tried to measure CEA, SLX, CA 19-9, CYFRA, ProGRP, and NSE in five samples for each tumor marker from patients with high serum values. However, none of those tumor markers could be detected in those breath condensates. Moreover, cytokines : IL-1beta, IL-6, IL-8 and TGF-beta were also not detected in the tested samples. We could detect prostanoids : PGE2 and LTB4 in several samples. But, the amounts were within the lowest range of detectable amount, and the measurements were neither reliable nor reproducible. We conclude that detection of high molecular weight substances such as tumor markers and cytokines in breath condensates were difficult. The detection of lower molecular weight substances such as prostanoids might be possible. However, more sensitive detection method or further concentration of breath condensates might be necessary for reliable results.

Research Products

• [Journal Article] All-trans retinoic acid modulates radiation-induced proliferation of lung fibroblasts via IL-6/IL-6R system (2006)
  • Author(s): Tabata C, Kubo H, Tabata R, Wada M, Sakuma K, Ichikawa M, Fujita S, Mio T, Mishima M
  • Journal Title: Am J Physiol Lung Cell Mol Physiol 290
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Prognosis of cough variant asthma : a retrospective analysis (2006)
  • Author(s): Matsumoto H, Niimi A, Takemura M, Ueda T, Tabuena R, Yamaguchi M, Matsuoka H, Hirai T, Muro S, Ito Y, Mio T, Chin K, Nishiyama H, Mishima M
  • Journal Title: J Asthma 43 Pages: 131-5
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] An N-glycan structure correlates with pulmonary metastatic ability of cancer cells. (2006)
  • Author(s): Sakuma K, Fujimoto I, Hitoshi S, Tanaka F, Ikeda T, Tanabe K, Toyokuni S, Wada H, Mio T, Mishima M, Ikenaka K
  • Journal Title: Biochem Biophys Res Commun. 340 Pages: 829-35
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Prognosis of cough variant asthma : a retrospective analysis (2006)
  • Author(s): Matsumoto H, Niimi A, Takemura M, Ueda T, Tabuena R, Yamaguchi M, Matsuoka H, Hirai T. Muro S, Ito Y, Mio T, Chin K, Nishiyama H, Mishima M.
  • Journal Title: J Asthma 2006 43(2)(in press) Pages: 131-135
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] An N-glycan structure correlates with pulmonary metastatic ability of cancer cells. (2006)
  • Author(s): Sakuma K, Fujimoto I, Hitoshi S, Tanaka F, Ikeda T, Tanabe K, Toyokuni S, Wada H, Mio T, Mishima M, Ikenaka K.
  • Journal Title: Biochem Biophys Res Commun.2006 Feb 17 (Epub 2005 Dec 20.) 340(3) Pages: 829-835
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] All-trans retinoic acid modulates radiation-induced proliferation of lung fibroblasts via IL-6/IL-6R system.L597 (2005)
  • Author(s): Tabata C, Kubo H, Tabata R, Wada M, Sakuma K, Ichikawa M, Fujita S, Mio T, Mishima M.
  • Journal Title: Am J Physiol Lung Cell Mol Physiol.2006 Mar (Epub 2005 Oct 28) 290(3)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Metastatic tumor in the right atrium (2004)
  • Author(s): Tabuena RP, Matsumoto H, Mio T, Nagata Y, Mishima M
  • Journal Title: Intern Med.2004 43 Pages: 445-6
  • NAID: 10013091208
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Metastatic tumor in right atrium. (2004)
  • Author(s): Tabuena RP, Matsumoto H, Mio T, Nagata Y, Mishima M.
  • Journal Title: Intern Med.2004 May 43(5) Pages: 445-446
  • NAID: 10013091208
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 呼吸器疾患治療のエビデンス (2005)
  • Author(s): 三尾直士
  • Publisher: 文光堂
  • Description: 「研究成果報告書概要(和文)」より