The investigation of the mechanisms and therapy of the abnormality in antidiuretic hormone action in patients with chronic renal failure
| Project/Area Number |
15590852
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
NONOGUCHI Hiroshi Kumamoto University, Dept of Nephrology, Graduate School of Medical Sciences, Associate professor, 大学院・医学薬学研究部, 助教授 (30218341)
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| Co-Investigator(Kenkyū-buntansha) |
TOMITA Kimio Kumamoto University, Dept of Nephrology, Professor, 大学院・医学薬学研究部, 教授 (40114772)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | antidiuretic hormone / V1a receptor / V2 receptor / V1a knockout mice / aquaporin 2 / metabolic acidosis / trafficking / 細胞内アシドーシス / 尿濃縮 / プロモーター / 集合尿細管 |
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| Research Abstract |
We investigated renal function and abnormality in V1a-knockout mice and found that there is no abnormality in urine concentrating ability. Vasopressin-dependent camp generation in V1a receptor-knockout mice was also not different from control mice. Now, we are investigating V1a receptor transgenic mice.
We next investigated urinary excretion of aquaporin-2(AQP2) in rats with metabolic acidosis. Although urinary AQP2 excretion was largely decreased, AQP2 mRNA and protein in outer medullary collecting ducts were increased in rats with metabolic acidosis. These data suggest the presence of abnormality in trafficking of AQP2 from intracellular vesicle to the apical membrane in metabolic acidosis. We observed same tendency in rats with potassium depletion. Since intracellular acidosis is known to occur in metabolic acidosis and potassium depletion, intracellular acidosis may have a key role for the decreased trafficking of AQP2 in the collecting ducts. We are now collaborating with Dr, Nielsen in Aarhus University in Denmark and Dr.knepper in National Institutes of health in USA.
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Report
(3 results)
Research Products
(29 results)
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[Journal Article] Mutations in human urate transporter 1 gene in presecretory reabsorption defect type of familial renal hypouricemia.2005
Author(s)
Wakida N, Tuyen do G, Adachi M, Miyoshi T, Nonoguchi H, Oka T, Ueda O, Tazawa M, Kurihara S, Yoneta Y, Shimada H, Oda T, Kikuchi Y, Matsuo H, Hosoyamada M, Endou H, Otagiri M, Tomita K, Kitamura K.
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Journal Title
J Clin Endocrinol Metab. 90
Pages: 2169-2174
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Intravenous iron administration induces oxidation of serum albumin in hemodialysis patients.2004
Author(s)
Anraku M, Kitamura K, Shinohara A, Adachi M, Suenga A, Maruyama T, Miyanaka K, Miyoshi T, Shiraishi N, Nonoguchi H, Otagiri M, Tomita K.
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Journal Title
Kidney Int. 66
Pages: 841-848
Description
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[Journal Article] Two male siblings with hereditary renal hypouricemia and exercise-induced ARF.2003
Author(s)
Tanaka M, Itoh K, Matsushita K, Matsushita K, Wakita N, Adachi M, Nonoguchi H, Kitamura K, Hosoyamada M, Endou H, Tomita K.
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Journal Title
Am J Kidney Dis. 42
Pages: 1287-1292
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Angiotensin-converting enzyme inhibitor withdrawal and ACE gene polymorphism.2003
Author(s)
Nonoguchi H, Kiyama S, Inoue H, Nakayama Y, Inoue T, Kohda Y, Machida K, Tajima A, Kitamura K, Miyoshi T, Shimada H, Shimada H, Tajiri M, Honda Y, Tanaka M, Tomita K.
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Journal Title
Clin Nephrol. 60
Pages: 225-232
Description
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