THE ANALYSIS OF ADENOVO GENE IS EXPRESSED WITH SECONDARY HYPERPARATHYROIDISM
| Project/Area Number |
15590856
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
NEGI Shigeo WAKAYAMA MEDICAL UNIVERSITY, MEDICAL DEPARTMENT, ASSOCIATE PROFESSOR, 医学部, 講師 (20208284)
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| Co-Investigator(Kenkyū-buntansha) |
AKIZAWA Tadao WAKAYAMA MEDICAL UNIVERSITY, MEDICAL DEPARTMENT, PROFESSOR, 医学部, 教授 (40102339)
HATAMURA Ikuji WAKAYAMA MEDICAL UNIVERSITY, MEDICAL DEPARTMENT, ASSISTANT PROFESSOR, 医学部, 助手 (80336883)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Secondary hyperparathyroidism / Parathyroid hormone / parathyroid-specific gene / chief cell / Suppression Subtractive Hybridization / Vitamin D analog / in situ hybridization / in situ hybridization法 / Supression Substractive hybrization法 / クローニング |
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| Research Abstract |
1.We performed the cloning of a new tissue-specific gene in human glands with secondary hyperparathyroidism using Suppression Subtractive Hybridization(SSH) method and determined the each primary structure of the new tissue-specific gene in homo sapiens, Rattus norvegicus and Mus musculus.
2.In situ hybridization, the chief cells in human parathyroid glands with secondary hyperparathyroidism expressed mRNA of the new tissue-specific gene.
3.The strong expression of the new tissue-specific gene can be seen to be expressed in chief cells in human parathyroid glands with secondary hyperparathyroidism.
4.Direct injection of Vitamin D analog into parathyroid gland, were 5/6 nephrectomized and fed a high-phosphorus, upregulated decreased CaR and VDR, and suppressed the proliferation of parathyroid cells and the expression of mRNA of the new tissue-specific gene.
These results suggest that the new tissue-specific gene is giving the proliferation of parathyroid cells with secondary hyperparathyroidism some influences.
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Report
(3 results)
Research Products
(6 results)
