THE ANALYSIS OF ADENOVO GENE IS EXPRESSED WITH SECONDARY HYPERPARATHYROIDISM
| Project/Area Number | 15590856 |
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Kidney internal medicine
|
|---|
| Research Institution | Wakayama Medical University |
|---|
Principal Investigator |
NEGI Shigeo WAKAYAMA MEDICAL UNIVERSITY, MEDICAL DEPARTMENT, ASSOCIATE PROFESSOR, 医学部, 講師 (20208284)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AKIZAWA Tadao WAKAYAMA MEDICAL UNIVERSITY, MEDICAL DEPARTMENT, PROFESSOR, 医学部, 教授 (40102339)
HATAMURA Ikuji WAKAYAMA MEDICAL UNIVERSITY, MEDICAL DEPARTMENT, ASSISTANT PROFESSOR, 医学部, 助手 (80336883)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed(Fiscal Year 2004)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost : ¥3,500,000)
Fiscal Year 2004 : ¥1,500,000 (Direct Cost : ¥1,500,000)
Fiscal Year 2003 : ¥2,000,000 (Direct Cost : ¥2,000,000)
|
|---|
| Keywords | Secondary hyperparathyroidism / Parathyroid hormone / parathyroid-specific gene / chief cell / Suppression Subtractive Hybridization / Vitamin D analog / in situ hybridization / in situ hybridization法 / Supression Substractive hybrization法 / クローニング |
|---|
| Research Abstract |
1.We performed the cloning of a new tissue-specific gene in human glands with secondary hyperparathyroidism using Suppression Subtractive Hybridization(SSH) method and determined the each primary structure of the new tissue-specific gene in homo sapiens, Rattus norvegicus and Mus musculus.
2.In situ hybridization, the chief cells in human parathyroid glands with secondary hyperparathyroidism expressed mRNA of the new tissue-specific gene.
3.The strong expression of the new tissue-specific gene can be seen to be expressed in chief cells in human parathyroid glands with secondary hyperparathyroidism.
4.Direct injection of Vitamin D analog into parathyroid gland, were 5/6 nephrectomized and fed a high-phosphorus, upregulated decreased CaR and VDR, and suppressed the proliferation of parathyroid cells and the expression of mRNA of the new tissue-specific gene.
These results suggest that the new tissue-specific gene is giving the proliferation of parathyroid cells with secondary hyperparathyroidism some influences.
|
Report
(3results)
Research Products
(6results)
