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THE ANALYSIS OF ADENOVO GENE IS EXPRESSED WITH SECONDARY HYPERPARATHYROIDISM

Research Project

Project/Area Number15590856
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionWakayama Medical University

Principal Investigator

NEGI Shigeo  WAKAYAMA MEDICAL UNIVERSITY, MEDICAL DEPARTMENT, ASSOCIATE PROFESSOR, 医学部, 講師 (20208284)

Co-Investigator(Kenkyū-buntansha) AKIZAWA Tadao  WAKAYAMA MEDICAL UNIVERSITY, MEDICAL DEPARTMENT, PROFESSOR, 医学部, 教授 (40102339)
HATAMURA Ikuji  WAKAYAMA MEDICAL UNIVERSITY, MEDICAL DEPARTMENT, ASSISTANT PROFESSOR, 医学部, 助手 (80336883)
Project Period (FY) 2003 – 2004
Project Status Completed(Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost : ¥3,500,000)
Fiscal Year 2004 : ¥1,500,000 (Direct Cost : ¥1,500,000)
Fiscal Year 2003 : ¥2,000,000 (Direct Cost : ¥2,000,000)
KeywordsSecondary hyperparathyroidism / Parathyroid hormone / parathyroid-specific gene / chief cell / Suppression Subtractive Hybridization / Vitamin D analog / in situ hybridization / in situ hybridization法 / Supression Substractive hybrization法 / クローニング
Research Abstract

1.We performed the cloning of a new tissue-specific gene in human glands with secondary hyperparathyroidism using Suppression Subtractive Hybridization(SSH) method and determined the each primary structure of the new tissue-specific gene in homo sapiens, Rattus norvegicus and Mus musculus.
2.In situ hybridization, the chief cells in human parathyroid glands with secondary hyperparathyroidism expressed mRNA of the new tissue-specific gene.
3.The strong expression of the new tissue-specific gene can be seen to be expressed in chief cells in human parathyroid glands with secondary hyperparathyroidism.
4.Direct injection of Vitamin D analog into parathyroid gland, were 5/6 nephrectomized and fed a high-phosphorus, upregulated decreased CaR and VDR, and suppressed the proliferation of parathyroid cells and the expression of mRNA of the new tissue-specific gene.
These results suggest that the new tissue-specific gene is giving the proliferation of parathyroid cells with secondary hyperparathyroidism some influences.

Report

(3results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report

Research Products

(6results)

All 2005 2004 2003

All Journal Article

  • [Journal Article] Biochemical and Cellular Effects Direct Maxcalcitol Injection Parathyroid Gland in Uremic Rats.2005

    • Author(s)
      Shiizaki K
    • Journal Title

      J Am Soc Nephrol. 16

      Pages : 97-108

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Biochemical and Cellular Efects Direct Maxcalcitol Injection ParathyroidGland in Uremic Rats.2005

    • Author(s)
      Shiizaki K, Negi, S, Hatamura I, Akizawa T et al.
    • Journal Title

      J Am Soc Nephrol 16

      Pages : 97-108

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Calcimimetic compound upregulates decreased calcium-sensing receptor expression level in parathyroid glands of rats with chronic renal insufficiency.2004

    • Author(s)
      Mizobuchi M
    • Journal Title

      J Am Soc Nephrol. 15

      Pages : 2579-2587

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Calcimimetic compound upregulates decreased calcium-sensing receptor expression level in parathyroid glands of rats with chronic renal insufficiency2004

    • Author(s)
      Mizobuchi M, Hatamura I, Negi S, Akizawa T. et al.
    • Journal Title

      J Am Soc Nephrol 15

      Pages : 2579-2587

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Percutaneous maxacalcitol injection therapy regresses hyperplasia of parathyroid and induces apoptosis in uremia2003

    • Author(s)
      Shiizaki K
    • Journal Title

      Kidney Int. 64(3)

      Pages : 992-1003

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Percutaneous maxacalcitol injection therapy regresses hyperplasia of parathyroid and induces apoptosis in uremia.2003

    • Author(s)
      Shiizaki K, Hatamura I, Negi, S, Akizawa T. et al.
    • Journal Title

      Kidney Int.Sep 64(3)

      Pages : 992-1003

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary

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Published : 2003-04-01   Modified : 2016-04-21  

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