Proteomic analysis of axonal injury in multiple sclerosis

• Project/Area Number: 15590894
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: KYUSHU UNIVERSITY
• Principal Investigator: MURAI Hiroyuki Kyushu University, University Hospital, Assistant professor, 大学病院, 講師 (80325464)
• Co-Investigator(Kenkyū-buntansha): ARAKI Norie Kumamoto University, Faculty of medicine, Assistant professor, 医学部, 講師 (80253722) ; IKEZOE Koji Kyushu University, University Hospital, Assistant professor, 大学病院, 講師 (80343317)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: multiple sclerosis / autoantibody / axonal injury / two-dimension immunoblot / proteomic analysis

Research Abstract

Pathologically, brain from multiple sclerosis (MS) patients show demyelination as well as axonal injury. Recently, it is indicated that axonal damage is profoundly related to the residual neurological deficit. Therefore, mechanism of axonal damage in MS has attracted a considerable attention. We applied proteomic analysis to identify the protein that is related to axonal damage of MS. Patients having conventional MS, optico-spinal MS, chronic cortical inflammation were screened for anti-neuronal antibody by single dimension immunoblotting. Patients who showed positive result was appled to two-dimension electrophoresis (2-DE). 2-DE was carried out in a horizontal electrophoresis system, for the first dimensional isoelectric focusing using Immobiline dry strip and by a Giant-slab gel electrophoresis system for the second-dimensional SDS-PAGE. The protein spots on the gels were electroblotted onto a polyvinylidene di£luoride (PVDF) membrane with a semi-dry blotting apparatus. The PVDF membrane was stained with 0.1% Coomassie Brilliant Blue (CBB). The stained gels and PVDF membranes were scanned and analyzed. Mass analysis revealed that one of the spot was fascin, which is a protein which connect axon to the oligodendroglia. This is one of the mechanisms that may playa role in developing axonal injury in MS.

Research Products

• [Journal Article] Th1 shift in CIDP versus Th2 shift in vasculitic neuropathy in CSE (2005)
  • Author(s): Mei F, Ishizu T, Murai H, Osoegawa M, Minohara M, Zhang K, Kira J
  • Journal Title: J Neurol Sci 228 Pages: 75-85
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Platelet-activating factor receptor gene polymorphism in Japanese patients with multiple sclerosis (2005)
  • Author(s): Osoegawa M, Miyagishi R, Ochi H, Nakamura I, Niino M, Kikuchi S, Murai H, Fukazawa T, Minohara M, Tashiro K, Kira J
  • Journal Title: J Neuroimmunol 161 Pages: 195-8
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Intrathecal activation of the IL-17/IL-8 axis tin opticospinal multiple sclerosis (2005)
  • Author(s): Ishizu T, Osoegawa M, Mei F, Kikuhi H, Tanaka M, Takakura Y, Minohara M, Murai H, Mihara F, Taniwaki T, Kira J
  • Journal Title: Brain 128 Pages: 988-1002
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Th1 shift in CIDP versus Th2 shift in vasculitic neuropathy in CSF. (2005)
  • Author(s): Mei F, Ishizu T, Murai H, Osoegawa M, Minohara M, Zhang K, Kira J
  • Journal Title: J Neurol Sci 228 Pages: 75-85
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Intrathecal activation of the IL-17/IL-8 axis in opticospinal multiple sclerosis. (2005)
  • Author(s): Ishizu T, Osoegawa M, Mei F, Kikuchi H, Tanaka M, Takakura Y, Minohara M, Murai H, Mihara F, Taniwaki T, Kira J
  • Journal Title: Brain 128 Pages: 988-1002
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Th1 shift in CIDP versus Th2 shift in vasculitic neuropathy in CSF (2005)
  • Author(s): Mei F, Ishizu T, Murai H, Osoegawa M, Minohara M, Zhang K, Kira J
  • Journal Title: J Neurol Sci 228 Pages: 75-85
• [Journal Article] Intrathecal activation of the IL-17/IL-8 axis in opticospinal multiple sclerosis (2005)
  • Author(s): Ishizu T, Osoegawa M, Mei F, Kikuchi H, Tanaka M, Takakura Y, Minohara M, Murai H, Mihara F, Taniwaki T, Kira J
  • Journal Title: Brain (in press)
• [Journal Article] effect of immunotherapy in myelitis with atopic diathesis (2004)
  • Author(s): Murai H, Arahata H, Osoegawa M, Ochi H, Minohara M, Taniwaki T, Tobimatsu S, Mihara F, Tsuruta Y, Inaba S, Kira J
  • Journal Title: J Neurol Sci 227 Pages: 39-47
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] High frequency of allergic conjunctivitis in myasthenia gravis without thymoma (2004)
  • Author(s): Murai H, Osoegawa M, Ochi H, Kira J
  • Journal Title: J Neurol Sci 225 Pages: 27-31
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effect of immunotherapy in myelitis with atopic diathesis. (2004)
  • Author(s): Murai H, Arahata H, Osoegawa M, Ochi H, Minohara M, Taniwaki T, Tobimatsu S, Mihara F, Tsuruta Y, Inaba S, Kira J
  • Journal Title: J Neurol Sci 227 Pages: 39-47
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High frequency of allergic conjunctivitis in myasthenia gravis without thymoma. (2004)
  • Author(s): Mural H, Osoegawa M, Ochi H, Kira J
  • Journal Title: J Neurol Sci 225 Pages: 27-31
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Platelet-activating factor acetylhydrolase gene polymorphism and its activity in Japanese patients with multiple sclerosis. (2004)
  • Author(s): Osoegawa M, Niino M, Ochi H, Kikuchi S, Murai H, Fukazawa T, Minohara M, Tashiro K, Kira J
  • Journal Title: J Neuroimmunol 150 Pages: 150-156
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of immunotherapy in myelitis with atopic diathesis (2004)
  • Author(s): Murai H, Arahata H, Osoegawa M, Ochi H, Minohara M, Taniwaki T, Tobimatsu S, Mihara F, Tsuruta Y, Inaba S, Kira J
  • Journal Title: J Neurol Sci 227 Pages: 39-47
• [Publications] 三野原元澄, 川尻真和, 孫暁嘉, 石津尚明, 小副川学, 村井弘之, 吉良潤一: "Subcortico-optico-myelitis with anti-neuronal autoantibody"神経免疫学. 12. 76-76 (2004)
• [Publications] 孫暁嘉, 荒木令江, 三野原元澄, 高倉由佳, 村井弘之, 佐谷秀行, 吉良潤一: "慢性進行性大脳皮質炎症例の脳自己抗原の二次元免疫ブロッティング法による解析"神経免疫学. 12. 77-77 (2004)
• [Publications] 村井弘之, 梅風君, 小副川学, 越智博文, 吉良潤一: "CIDPの髄液CD4陽性T細胞内サイトカインの解析"末梢神経. 14. 147-149 (2003)
• [Publications] Ochi H, Murai H, Osoegawa M, Minohara M, Inaba S, Kira J: "Juvenile muscular atrophy of distal upper extremity associated with airway allergy : Two cases successfully treated by plasma exchange"J Neurol Sci. 206. 109-114 (2003)
• [Publications] Osoegawa M, Ochi H, Kikuchi H, Shirabe S, Nagashima T, Tsumoto T, Tamura Y, Yamabe K, Takahashi H, Iwaki T, Kira J: "Eosinophilic myelitis associated with atopic diathesis : a combined neuroimaging and histopathological study"Acta Neuropathol (Berl). 105. 289-295 (2003)