Detailed mechanisms of signal transduction pathways of AngII in chromaffin cells
| Project/Area Number |
15590967
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | UNIVERSITY OF TSUKUBA |
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Principal Investigator |
TAKEKOSHI Kazuhiro UNIVERSITY OF TSUKUBA, Graduate School of Comprehensive Human Sciences, Assistant Professor, 大学院・人間総合科学研究科, 助教授 (40261804)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Ang II / AT_1 / AT_2 / MAPK / PKC / JNK / P38 / AngII受容体 / AT_1受容体 / AT_2受容体 / DNAチップ / 情報伝達経路 / ERK / 副腎髄質 / カテコールアミン / 発現プロファイリング / Ang II |
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| Research Abstract |
Two distinct types of angiotensin II (AngII) receptors, AT_1 and AT_2, have been cloned. We have previously shown that stimulation of AT_2 reduces intracellular cGMP levels in cultured porcine chromaffin cells in which AT_2 is the predominantly expressed receptor. However, it has not been determined whether AT_1 or AT_2 affects signal transduction pathways in chromaffin cells. Using Genetic Micro System(GMS-418-array scanner), we compare the effects of AngII plus CV-11974 (an AT_1 antagonist, which simulates specific AT_2 stimulation) with AngII plus PD 123319 (an AT_2 antagonist, which simulates specific AT_1 stimulation). There are several genes down and over expressed under such condition. We are characterize these genes.
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Report
(4 results)
Research Products
(8 results)
