| Project/Area Number |
15591107
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kobe University |
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Principal Investigator |
KAWASAKI Keiichiro Kobe University, Hospital, Assistant, 医学部附属病院, 助手 (30359864)
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| Co-Investigator(Kenkyū-buntansha) |
TAKESHIMA Yasuhiro Kobe University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (40281141)
MATSUO Masafumi Kobe University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (10157266)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | childhood leukemia / chimera mRNA / cloning |
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| Research Abstract |
Though the analyze of pediatric malignant diseases have made great progress in recent years and we can reveal molecular mechanism of several diseases, the character and mechanism of cells of acute megakaryocytic leukemia (AMKL) with t(1;9) is still unclear. We planned the study of cloning of chimera mRNA of the leukemic cell of AMKL because the objectively elucidation of the cause of AMKL is one of the very important problem of pediatric hematology and oncology. At the same time we experienced two cases; one is a boy with acute myelocytic leumemia (AML) with t(15;17) and the other is a boy with synovial sarcoma with t(2;2). Very interestingly, the points of translocation are 15q13 and 17q11 which are differ from the point of translocation of acute promyelocytic leukemia well known as PML/RARA. The leukemic cell of this case of AML is actually the type of M2 morphologically and we diagnosed very hardly. Although this case of AML could be induced in the first remission smoothly and underwent five courses of consolidation chemotherapy, the relapse occurred immediately after the last course of the consolidation chemotherapy. He could not be induced in the second remission and received allogeneic bone marrow transplantation from HLA-matched sibling notwithstanding on disease. The second relapse occurred after one month from transplantation. The point of translocation of the case of synovial sarcoma is 2q35 which is the same as PAX3 which is often seen in the alveolar rhabdomyosarcoma with t(2;13)and PAX3/FKHR chimera mRNA. We also plan the study of cloning of chimera mRNA of these two cases and sampled genome DNA of the leukemic/tumor cells and made the genome library. At last, applying the known arrangement, we obtained the production as chimera mRNA by 5'RACE method.
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