Purification, expansion, and reconstitution of mesodermal stemcells from human bone marrow
| Project/Area Number |
15591111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
KOBAYASHI Masao Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (00162016)
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Takashi Hiroshima University, Hospital, Assistant Professor, 病院・講師 (90271064)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Mesenchymal Stem Cell / Bone Marrow / Hematopoiesis / ABC-G2 / FACS / Mesenchymal Stem Cell / Bone Marrow / 免疫不全マウス / ABC-G2 / 異種間移植 |
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| Research Abstract |
Mesenchymal stem Cells were purified from human bone marrow cells based on the expression of CD34,CD38,CD45, anti-glycophorin A, and ABCG2 antibodies by FACS vantage. The frequency of CD45-negative and ABCG2-positive cells in bone marrow was less than 0.01%. The purified CD45-negative and ABCG2-positive cells were cultured under low serum and low glucose medium with appropriate growth factors resulting in the differentiation into bone, chondrocyte, and adipocytes. Furthermore, bone marrow cells were transplanted into immunodeficient mice (NOD/SCID/b2-microglobulin^<null>) to confirm the reconstituting ability in different organs. The cells negative for ABCG2 were reconstiuted as hematopoietic cells differentiating into leukocytes, whereas those positive for ABCG2 migrated into mesodermal tissues. These observations suggest that the expression of ABCG2 may distinguish the origin between hematopoietic stem cells and mesenchymal stem cells.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Dysregulation of transcriptions in primary granule constituents during myeloid proliferation and differentiation in patients with severe congenital neutropenia.2003
Author(s)
Kawaguchi H, Kobayashi M, Nakamura N, Konishi N, Miyagawa S, Sato T, Toyota H, Komada Y, Kojima S, Todoroki Y, Ueda K, Katoh O
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Journal Title
Journal of Leukocyte Biology 73
Pages: 225-234
Description
「研究成果報告書概要(欧文)」より
Related Report
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