Establishing of cell strain from mouse ES cells for the therapeutic use and study in neurodegenerative disorders
| Project/Area Number |
15591125
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Osaka City University |
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Principal Investigator |
TANAKA Akemi Osaka City University, Department of Pediatrics, Associate Professor, 大学院・医学研究科, 助教授 (30145776)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Mitsuyo Osaka City University, Department of Anatomy and Neurobiology, Lecturerer, 大学院・医学研究科, 講師 (40122080)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | ES cell / glial precursor cell / Cell therapy / Lysosomal storage disease / β-Galactosidase knock-out mouse / Neurodegenerative disorder |
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| Research Abstract |
The study was carried out for treating the brain of neurodegenerative disorders by transplanting cells to deliver the deficit materials.
At first, we established ES cell-derived glial precursor cells. ES cells were grown to be embryoid bodies when cultured with insulin, transferrin, and fibronectin, in the absence of LIF. The cells were spread by trypsinization and continued the culture with insulin, transferrin, progesterone, putrescine, and laminin. The cells became bipolar cells when basic fibroblast growth factor (FGF2) and epidermal growth factor (EGF) were added. The cells were stained immunochemically with anti-A2B5, anti-GFAP, and anti-04 antibodies, and confirmed the cell-differentiations.
The ES cell-derived glial precursor cells were transplanted into the brain ventricle of β-galactosidase knock-out mouse at 2 weeks of age. One week after the transplantation, the mice were sacrificed and the brain specimens were stained with X-Gal. However, no X-Gal positive cells were found in the brain. When the cells were transplanted in the ventricle of newborn mice, a few X-Gal positive cells were clotted in the brain at one week after the plantation.
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] Non-invasive mass screening method for Fabry disease by measuring globotriasylceramide in whole urine samples using tandem mass spectrometry.2005
Author(s)
Kitagawa T, Ishige N, Suzuki K, Owada M, Ohashi T, Kobayashi M, Eto Y, Tanaka A, Mills K, Winchester B, Keutzer J
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Journal Title
Molecular Genetetics and Metabolism (In press)
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Non-invasive mass screening method for Fabry disease by measuring globotriasylceramide in whole urine samples using tandem mass spectrometry.
Author(s)
Kitagawa T, Ishige N, Suzuki K, Owada M, Ohashi T, Kobayashi M, Eto Y, Tanaka A, Mills K, Winchester B, Keutzer J
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Journal Title
Molecular Genet Metabolism (in press)
Description
「研究成果報告書概要(欧文)」より
Related Report
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