| Project/Area Number |
15591188
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
YAMAMOTO Yuki Wakayama Medical University, Department of Dermatology, Assistant prof., 医学部, 講師 (90316117)
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| Co-Investigator(Kenkyū-buntansha) |
FURUKAWA Fukumi Wakayama Medical University, Department of Dermatology, Prof.& chairman, 教授 (40156964)
OTANI Toshio Wakayama Medical University, Department of Dermatology, Instructor, 講師 (10326366)
KISHI Tomoo Wakayama Medical University, Department of Dermatology, Instructor, 助手 (10336878)
NISHIDE Takeshi Wakayama Medical University, Department of Dermatology, Instructor, 助手 (70347590)
上出 康二 和歌山県立医科大学, 医学部, 助教授 (50176608)
山本 有紀 和歌山県立医科大学, 医学部, 助手 (90316117)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | trichloroacetic acid / phenol / apoptosis / TUNEL method / caspase-3 / chemical peeling / Skin cancer / p-53 |
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| Research Abstract |
1. In view of wound healing in trichloroacetic acid(TCA) or phenol treated skin, we studied the expression patterns of keratin 6, 7, 10 and 16 and the immunohistochemical distribution of human beta-1 integrin and tenascin. In our result, histological changes of epidermis in TCA treated skin and phenol treated skin were very similar in the hematoxyline-eosin stained paraffin sections, but shows that the immunohistochemical stainings were differed. However, in the dermis phenol penetrated into the skin quickly and induced changes in endothelial cells and the phenol peels might differ from histological effects of TCA peels. In tissues damaged with phenol, skin degenerations were positively detected by a TUNEL method and expression of activated Caspase-3. However, we did not observe up-regulation of p53 or Fas. Meanwhile TCA peeled skins showed positive reaction by a TUNEL method, but not by Caspase-3 activation, Fas, or p53. These findings suggest that skin degenerations after phenol peels will lead to apoptosis via Caspase-3 activation, which differs from skin degenerations after TCA peels.
2. In aged patients with skin tumor who refused the operation or had the difficulty in the surgery, we used TCA and/or phenol peels as a treatment and followed the clinical and histological course of them for one to 4 years. In the result we thought complete cure with TCA and/or phenol peels were expected for actinic keratosis(AK), Bowen's disease and basal cell carcinoma(BCC) of histologically superficial type. Some patients (2 AK and 2 Bowen's disease) were resistant to the therapy. The tumor cells in these patients showed strongly expression of p-53 during the course of the treatment.
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