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Amygdalic levels of monoamines, amino acids and nitric oxide rise upon exposure to conditioned fear stress without elevation of amino acids

Research Project

Project/Area Number 15591242
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionKitasato University, School of Medicine

Principal Investigator

SUZUKI Eiji  Kitasato University, School of Medicine, Assistant Professor, 医学部, 講師 (60226496)

Co-Investigator(Kenkyū-buntansha) MIYAOKA Hitishi  Kitasato University, School of Medicine, Professor, 医学部, 教授 (40209862)
TAKAHASHI Megumi  Kitasato University, School of Medicine, Assistant Professor, 医学部, 講師 (00305476)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordsconditioned fear stress / Interferon-alpha / monoamine / amino acid / amygdala / nitric oxide / lithium / imipramine / 脳マイクロダイアライシス / 恐怖条件づけ / 神経伝達物質
Research Abstract

Conditioned fear is an artificial stress, induced by a stimulus, such as a tone, that does not elicit fear in nature. The amygdala is considered to be the area involved in acquisition, consolidation and recall of fear. The present study was designed to continuously monitor changes in extracellular levels of glutamate, NO_3^-, dopamine and serotonin in the amygdala, at the acquisition of conditioned fear on Day 1 and at fear recall in response to a tone as a conditioned stimulus on Day 2, using the in vivo microdialysis method. Glutamate was elevated only on Day 1, while dopamine, serotonin and NO_3^- rose on both days. Using a in-vivo brain microdialysis method, in addition, we investigated effects of intraperitoneal administration with lithium, imipramine, and diazepam on NO_3^- levels in the rat amygdala. Lithium significantly declined, but that of imipramine raised NO_3^- levels as compared with control. These suggest that lithium and imipramine induce contrastive effects on nitric oxide related systems in the brain. We also investigated the influence of peripherally administered interferon-alpha on amino acid levels in the brain. One day of interferon-alpha treatment induced no significant changes in any of these amino acids. After 4 days of interferon-alpha injections, glutamate, glycine, taurine and gamma-aminobutyric acid levels were significantly higher than those in the control frontal cortex, striatum, hippocampus, amygdala, and thalamus. However, most of these amino acids returned to approximately basal levels, or even lower, with 14-day-treatment.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (31 results)

All 2005 2004 2003 2002 Other

All Journal Article (25 results) Publications (6 results)

  • [Journal Article] Effects of intraperitoneal administration of interferon -alpha for one, four and fourteen days on amino acid levels in various rat brain regions.2005

    • Author(s)
      Yokoyama M, Suzuki E, Sato T, Maruta S, Inada K, Watanabe S, Miyaoka H.
    • Journal Title

      Journal of interferon and Cytokine research 25(in press)(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Amygdalic levels of dopamine and serotonin rise upon exposure to conditioned four stress without elevation of glutamate.2005

    • Author(s)
      Yokoyama M, Suzuki E, Sato T, Maruta S, Inada K, Watanabe S, Miyaoka H.
    • Journal Title

      Neuroscience letters (in press)(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of intraperitoneally injection lithium, impramine and diazepam on nitrate levels in rat amygdala.2005

    • Author(s)
      Maruta S, Suzuki E, Yokoyama M, Sato T, Inada K, Watanabe S, Miyaoka H.
    • Journal Title

      Psychiatry and clinical neuroscience (in press)(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Aconiti tuber increases plasma nitrite and nitrate levels in humans.2005

    • Author(s)
      Yamada K, Suzuki E, Nakaki T, Watanabe S, Kanba S
    • Journal Title

      Journal of ethnopharmacology 96

      Pages: 165-169

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Depression and associated factors of informal caregivers versus professional caregivers of demented patients.2005

    • Author(s)
      Megumi Takahashi, Katsutoshi Tanaka, Hitoshi Miyaoka
    • Journal Title

      Psychiatry and Clinical Neuroscience (in press)(印刷中)

    • NAID

      10018029491

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Daily omega-3 fatty acid intake and depression in Japanese patients with newly diagnosed lung cancer.2004

    • Author(s)
      Suzuki S, Akechi T, Kobayashi M, Taniguchi K, Goto K, Sasaki S, Tsugane S, Nishiwaki Y, Miyaoka H, Uchitomi Y.
    • Journal Title

      British Journal of Cancer Feb23;90(4)

      Pages: 787-793

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Nitric oxide involvement in depression during interferon-alpha therapy2003

    • Author(s)
      Eiji Suzuki, Yoshiko Yoshida, Akitaka Shibuya, Hitoshi Miyaoka
    • Journal Title

      International Journal of Neuropsychopharmacology 6

      Pages: 415-419

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of a high-sucrose diet on body weight, plasma triglycerides, and stress tolerance2003

    • Author(s)
      Masao Kanazawa, Chang Young Xue, Haruaki Kageyama, Eiji Suzuki, Rokuro Ito, Yoshio Namba, Toshimasa Osaka, Shuichi Kimura, Shuji Inoue
    • Journal Title

      Nutrition Reviews 61

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Plasma nitrate levels in deficit vs. nondeficit forms of schizophrenia2003

    • Author(s)
      Eiji Suzuki, Toshio Nakaki, Makoto Nakamura, Hitoshi Miyaoka
    • Journal Title

      Journal of Psychiatry & Neuroscience 28

      Pages: 288-292

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nitric oxide involvement in depression during interferon-alpha therapy.2003

    • Author(s)
      Eiji Suzuki, Yoshiko Yoshida, Akitaka Shibuya, Hitoshi Miyaoka
    • Journal Title

      International Journal of Neuropsychopharmacology 6(4)

      Pages: 415-419

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of a high-sucrose diet on body weight, plasma triglycerides, and stress tolerance.2003

    • Author(s)
      Masao Kanazawa, Chang Young Xue, Haruaki Kageyama, Eiji Suzuki, Rokuro Ito, Yoshio Namba, Toshimasa Osaka, Shuichi Kimura, Shuji Inoue
    • Journal Title

      Nutrition Reviews 61(5 Pt 2)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Plasma nitrate levels in deficit vs. nondeficit forms of schizophrenia.2003

    • Author(s)
      Eiji Suzuki, Toshio Nakaki, Makoto Nakamura, Hitoshi Miyaoka
    • Journal Title

      Journal of Psychiatry & Neuroscience 28(4)

      Pages: 288-292

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nitric oxide induced heat shock protein 70 mRNA in rat hypothalamus during acute restraint stress under sucrose diet.2003

    • Author(s)
      Eiji Suzuki, Haruaki Kageyama, Toshio Nakaki, Shigenobu Kanba, Shuji Inoue, Hitoshi Miyaoka
    • Journal Title

      Cellular and Molecular Neurobiology 23(6)

      Pages: 953-962

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Antisense hippocampal knockdown of NMDA-NR1 by HVJ-liposome vector induces deficit of prepulse inhibition but not of spatial memory.2003

    • Author(s)
      Ken Inada, Jun Ishigooka, Takeshi Anzai, Eiji Suzuki, Hitoshi Miyaoka, Makoto Saji
    • Journal Title

      Neuroscience Research 45(4)

      Pages: 473-481

    • NAID

      50000810245

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Plasma nitrate and nitrite levels of patients with eating disorders.2003

    • Author(s)
      Eiji Suzuki, Hiroko Mizushima, Yutaka Ono, Masamoto Yokoyama, Hitoshi Miyaoka
    • Journal Title

      Psychiatry and Clinical Neurosciences 57

      Pages: 343-343

    • NAID

      10018973742

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cytochrome P450 2D6 Polymorphism and Character Traits.2003

    • Author(s)
      Eiji Suzuki, Yoshie Kitao, Yutaka Ono, Yoshimi Iijima, Toshiya Inada
    • Journal Title

      Psychiatry Genetics 13(2)

      Pages: 111-113

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Antipsychotic, Antidepressant, Anxiolytic, and Anticonvulsant Drugs Induce Type II Nitric Oxide Synthase mRNA in Rat Brain.2002

    • Author(s)
      Eiji Suzuki, Toshio Nakaki, Futoshi Shintani, Shigenobu Kanba, Hitoshi Miyaoka
    • Journal Title

      Neuroscience Letters 333(3)

      Pages: 217-219

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Early-Onset Tardive Dyskinesia induced by Co-Administration of Risperidone and Anticholinergic Agent.2002

    • Author(s)
      Eiji Suzuki, Michiyuki Obata, Yoshiko Yoshida, Hitoshi Miyaoka
    • Journal Title

      American Journal of Psychiatry 159(11)

      Pages: 1948-1948

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Antipsychotic, Antidepressant, Anxiolytic, and Anticonvulsant Drugs Induce Type II Nitric Oxide Synthase mRNA in Rat Brain.2002

    • Author(s)
      Eiji Suzuki, Toshio Nakaki, Futoshi Shintani, Shigenobu Kanba, Hitoshi Miyaoka
    • Journal Title

      Neuruscience Letters 333(3)

      Pages: 217-219

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of intraperitoneal administration of interferon-alpha for one, four and fourteen days on amino acid levels in various rat brain regions.

    • Author(s)
      Masamoto Yokoyama, Suzuki Eiji, Taku Sato, Shuuji Maruta, Ken Inada, Shigeru Watanabe, Hitishi Miyaoka
    • Journal Title

      J.Interferon Cytokine Res (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Amygdalic levels of dopamine and serotonin rise upon exposure to conditioned fear stress without elevation of glutamate

    • Author(s)
      Masamoto Yokoyama, Suzuki Eiji, Taku Sato, Shuuji Maruta, Shigeru Watanabe, Hitishi Miyaoka
    • Journal Title

      Neurosci.Lett (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of intraperitoneally injection lithium, imipramine and diazepam on nitrate levels in rat amygdala

    • Author(s)
      Shuuji Maruta, Suzuki Eiji, Masamoto Yokoyama, Taku Sato, Ken Inada, Shigeru Watanabe, Hitishi Miyaoka
    • Journal Title

      Psychiatry and Clinical Neuroscience (in press)

    • NAID

      10018029006

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of intraperitoneal administration of interferon-alpha for one, four and fourteen days on amino acid levels in various rat brain regions.

    • Author(s)
      Masamoto Yokoyama, Suzuki Eiji, Taku Sato, Shuuji Maruta, Ken Inada, Shigeru Watanabe, Hitishi Miyaoka
    • Journal Title

      J.Interferon Cytokine Res. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Amygdalic levels of dopamine and serotonin rise upon exposure to conditioned fear stress without elevation of glutamate.

    • Author(s)
      Masamoto Yokoyama, Suzuki Eiji, Taku Sato, Shuuji Maruta, Shigeru Watanabe, Hitishi Miyaoka
    • Journal Title

      Neurosci.Lett. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of intraperitoneally injection lithium, imipramine and diazepam on nitrate levels in rat amygdala.

    • Author(s)
      Shuuji Maruta, Suzuki Eiji, Masamoto Yokoyama, Taku Sato, Ken Inada, Shigeru Watanabe, Hitishi Miyaoka
    • Journal Title

      Psychiatry and Clinical Neuroscience (in press)

    • NAID

      10018029006

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Eiji Suzuki, Yoshie Kitano, Yoshimi Iijima, Toshiya Inaba: "Cytochrome P450 2D6 Polymorphism and Character Traits"Psychiatry Genetics. 13巻・2号. 111-113 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Eiji Suzuki, Toshio Nakaki, Makoto Nakamura, Hitoshi Miyaoka: "Plasma Nitrate Levels in Deficit vs. Nondeficit Forms of Schizophrenia"Journal of Psychiatry & Neuroscience. 28巻・4号. 288-292 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ken Inada, Jun Ishigooka, Takashi Anzai, Eiji Suzuki, Hitoshi Miyaoka, Makoto Saji: "Antisense hippocampal knockdown of NMDA-NR1 by HVJ-liposome vector induces deficit of prepulse inhibition but not of spatial memory"Neuroscience Research. 45巻・4号. 473-481 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Eiji Suzuki, Haruaki Kageyama, Toshio Nakaki, Shigenobu Kanba, Shuji Inoue, Hitoshi Miyaoka: "Nitric Oxide induced Heat Shock Protein 70 mRNA in Rat Hypothalamus during Acute Restraint Stress under Sucrose Diet"Cellular and Molecular Neurobiology. 23巻・6号. 907-915 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki E., Nakaki T., Kanba S., Shintani F., Miyaoka H.: "Long-term imipramine treatment increases nitrate levels in the rat hypothalamus"Cellular and Molecular Neurobiology. 23巻・6号. 953-962 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Eiji Suzuki, Yoshiko Yoshida, Akitaka Shibuya, Hitoshi Miyaoka: "Nitric oxide involvement in depression during interferon-alpha therapy"International Journal of Neuropsychopharmacology. 6巻・4号. 415-419 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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