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A role of calcitonin receptor family on progression of breast cancer

Research Project

Project/Area Number 15591353
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionWakayama Medical University

Principal Investigator

NAKAMURA Misa (2004)  Wakayama Medical University, Pathology, Assistant Professor, 医学部, 講師 (70285386)

桜井 武雄 (2003)  和歌山県立医科大学, 医学部, 教授 (00073700)

Co-Investigator(Kenkyū-buntansha) YOSHIMURA Goro  Wakayama Medical University, Surgery, Associate Professor, 医学部, 助教授 (70201051)
UMEMURA Teiji  Wakayama Medical University, Surgery, Assistant Professor, 医学部, 助手 (90295824)
中村 美砂  和歌山県立医科大学, 医学部, 助手 (70285386)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsbreast cancer / calcitonin receptor / lymphatic node metastasis
Research Abstract

There is a growing body of evidence indicating that Calcitonin(CT) and its receptor(CTR) is involved in cell growth, differentiation and tissue development. Using laser capture microdissection(LCM) and real-time reverse transcription polymerase chain reactions(RT-PCR), we have investigated CTR mRNA expression in 60 primary breast cancers, including 14 pairs of matched cancers and unaffected ductal epithelia from the same patients. Our results demonstrate that CTR mRNA was constantly expressed in normal ductal epithelium and in breast cancer. In the 14 cases where matched samples were available, a decrease in CTR mRNA expression was found in 9 breast cancers (64.3%), an increased CTR expression in 2 cases (14.3%) and no significant change in 3 cases (21.4%). In 60 cases of primary breast cancers, decreased CTR expression was found in 44 (73.3%), increased CTR expression was detected in 10 cases (16.7%) and no change was observed in 6 cases (10%). Decreased CTR expression was found more … More often in cases with lymph node metastasis (p=0.0498) and lymphatic invasion (p=0.0179). Also there was a decreased CTR expression in cases with an extensive intraductal component (p=0.0543) and a high nuclear grade (p=0.1934), although this was not statistically significant. Overall, we conclude that CTR mRNA was constantly expressed in unaffected ductal epithelium, whereas decreased CTR mRNA expression was frequently found in breast cancers, particularly in cases with lymph node metastasis and lymphatic invasion.
MDA-MB-231 human breast cancer cells, which represent metastatic, ERK1/2 phosphorylated constitutively., CT inactivates c-Raf via the PKA pathway, resulting in suppression of Erk1/2 phosphorylation, and finally decreases uPA expression in MDA-MB-231 cells. CT caused a decrease in invasion of MDA-MB-231 cells in Matrigel invasion assays (P<0.05). These data suggested that breast cancer metastasis was at least partially attributable to activated ERK pathway, and CT suppresses activated ERK1/2 and inhibits invasiveness in MDA-MB-231 breast cancer cells. CTR might be of great potential significance in breast cancer progression. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (19 results)

All 2005 2004 2003 Other

All Journal Article (15 results) Publications (4 results)

  • [Journal Article] Osteoclast-like cells express receptor activity modifying protein 2 : application of laser capture microdissection.2005

    • Author(s)
      Nakamura M, Morimoto S, Yang Q, Nakamura Y, Mori I, Kakudo K.
    • Journal Title

      J Mol Endocrinol. 34(1)

      Pages: 257-261

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Angiotensin II AT2 receptor localization in cardiovascular tissues by its antibody developed in AT2 gene-deleted mice.2005

    • Author(s)
      Utsunomiya H, Nakamura M, Kakudo K, Inagami T, Tainura M.
    • Journal Title

      Regul Pept. 30:126(3)

      Pages: 155-161

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Osteoclast-like cells express receptor activity modifying protein 2 : application of laser capture microdissection2005

    • Author(s)
      Nakamura M, Morimoto S, Yang Q, Hisamatsu T, Hanai N, Nakamura Y, Mori I, Kakudo K.
    • Journal Title

      J Mol Endocrinol. 34(1)

      Pages: 257-261

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Angiotensin II AT2 receptor localization in cardiovascular tissues by its antibody developed in AT2 gene-deleted mice.2005

    • Author(s)
      Utsunomiya H, Nakamura M, Kakudo K, Inagami T, Tamura M.
    • Journal Title

      Regul Pept. 30;126(3)

      Pages: 155-161

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Localization of calcitonin receptor mRNA in rat kidney : an in situ hybridization study.2004

    • Author(s)
      Ishii A, Nakamura M, Nakamura A, Kimura M, Kakudo K
    • Journal Title

      Acta Histochemica et Cytochemica 37(4)

      Pages: 259-265

    • NAID

      110003144387

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Calcitonin receptor gene and breast cancer : quantitative analysis with laser capture microdissection.2004

    • Author(s)
      Wang X, Nakamura M, Mori I, Nakamura Y, Sakurai T, Kakudo K.
    • Journal Title

      Breast Cancer Res Treat. 83(2)

      Pages: 109-117

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] A modified rapid Papanicolaou stain for imprint smears.2004

    • Author(s)
      Sato M, Taniguchi E, Kagiya T, Nunobiki O, Yang Q, Nakamura M, Mori I, Kakudo K
    • Journal Title

      Acta Cytol 48(3)

      Pages: 461-462

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Calcitonin receptor gene and breast cancer : quantitative analysis with laser capture microdissection.2004

    • Author(s)
      Wang X, Nakamura M, Mori I, Takeda K, Nakamura Y, Utsunomiya H, Yoshimura G, Sakurai T, Kakudo K
    • Journal Title

      Breast Cancer Res Treat 83(2)

      Pages: 109-117

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A modified rapid Papanicolaou stain for imprint smears.2004

    • Author(s)
      Sato M, Taniguchi E, Kagiya T, Nakamura M, Mori I, Kakudo K.
    • Journal Title

      Acta Cytol. 48(3)

      Pages: 461-462

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Magnetic resonance axillography for preoperative diagnosis of the axillopectoral muscle (Langer's axillary arch) : a case report.2003

    • Author(s)
      Suzuma T, Sakurai T, Yoshimura G, Umemura T, Shimizu Y, Okamura Y.
    • Journal Title

      Breast Cancer 10(3)

      Pages: 281-283

    • NAID

      50000275229

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Anastrozole-induced remission of diaphragmatic paralysis from metastatic breast cancer treated with multiple prior therapies.2003

    • Author(s)
      Suzuma T, Sakurai T, Yoshimura G, Umemura T, Yang QF, Okamura Y.
    • Journal Title

      Breast Cancer 10(2)

      Pages: 153-156

    • NAID

      50000796564

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Flt-4-positive vessel density correlates with vascular endothelial growth factor-d expression, nodal status, and prognosis in breast cancer.2003

    • Author(s)
      Nakamura Y, Yasuoka H, Tsujimoto M, Yang Q, Imabun S, Nakahara M, Nakao K, Nakamura M, Mori I, Kakudo K.
    • Journal Title

      Clin Cancer Res. 9(14)

      Pages: 5313-5317

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinicopathological significance of vascular endothelial growth factor-C in breast carcinoma with long-term follow-up.2003

    • Author(s)
      Nakamura Y, Yasuoka H, Tsujimoto M, Yang Q, Tsukiyama A, Imabun S, Nakahara M, Nakao K, Nakamura M, Mori I, Kakudo K.
    • Journal Title

      Mod Pathol. 16(4)

      Pages: 309-314

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prognostic significance of vascular endothelial growth factor D in breast carcinoma with long-term follow-up.2003

    • Author(s)
      Nakamura Y, Yasuoka H, Tsujimoto M, Yang Q, Imabun S, Nakahara M, Nakamura M, Mori I, Kakudo K.
    • Journal Title

      Clin Cancer Res. 9(2)

      Pages: 716-721

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prognostic value of Bcl-2 in invasive breast cancer receiving chemotherapy and endocrine therapy.2003

    • Author(s)
      Yang Q, Sakurai T, Yoshimura G, Suzuma T, Umemura T, Nakamura M, Nakamura Y, Mori I, Kakudo K.
    • Journal Title

      Oncol Rep. 10(1)

      Pages: 121-125

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Wang, X., Nakamura, M., Mori, I., Takeda, K., Nakamura, Y., Utsunomiya, H., Yoshimura, G., Sakurai, T., Kakudo K.: "Calcitonin Receptor Gene and Breast Cancer : Quantitative Analysis with Laser Capture Microdissection"Breast Cancer Research and Treatment. 83. 109-117 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuma T, Sakurai T, Yoshimura G, Umemura T, Shimizu Y, Yang QF, Okamura Y: "Magnetic resonance axillography for preoperative diagnosis of the axillopectoral muscle (Langer's axillary arch) : a case report."Breast Cancer. 10(3). 281-283 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuma T, Sakurai T, Yoshimura G, Umemura T, Shimizu Y, Yang QF, Okamura Y: "Anastrozole-induced remission of diaphragmatic paralysis from metastatic breast cancer treated with multiple prior therapies."Breast Cancer. 10(2). 153-156 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yang Q, Sakurai T, Yoshimura G, Suzuma T, Umemura T, Nakamura M, Nakamura Y, Mori I, Kakudo K: "Prognostic value of Bcl-2 in invasive breast cancer receiving chemotherapy and endocrine therapy."Oncol Rep.. 10(1). 121-125 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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