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Intratumoral injection of dendritic cells after treatment of anticancer drugs induces tumor-specific antitumor effect in vivo

Research Project

Project/Area Number 15591412
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

TANAKA Fumiaki  Kyushu University, Kyushu Univ.Hospital, Research Associate, 大学病院, 助手 (30332836)

Co-Investigator(Kenkyū-buntansha) MORI Masaki  Kyushu University, Medical Institute of Bioregulation, Professor, 生体防御医学研究所, 教授 (70190999)
UTSUNOMIYA Tohru  Kyushu University, Kyushu Univ.Hospital, Assistant Professor, 大学病院, 講師 (30304801)
MIMORI Koshi  Kyushu University, Medical Institute of Bioregulation, Research Associate, 生体防御医学研究所, 助手 (50322748)
YOSHIKAWA Yasuji  Kyushu University, Kyushu Univ.Hospital, Associate Professor, 大学病院, 助教授 (80124816)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsAnticamcer drug / dendritic cells / Cryotherapy / killer T cells / CD8陽性細胞
Research Abstract

We have investigated the in vivo antitumor effects of intratumoral(i.t.) administration of dendritic cells(DC) following low-dose chemotherapy using cisplatin plus 5-FU. Combination of i.t.injection of DC and systemic chemotherapy induced complete rejection of the treated tumor, MC38 murine adenocarcinoma. Furthermore, the antitumor effects were also observed on a distanttumor inoculated in the contralateral flank of the animal. When the 10 times the number of tumor cells were inoculated, the antitumor effect of the combination of DC following chemotherapy was also confirmed, and in comparison to that of DC or chemotherapy alone, thereafter contributed to a greater prolongation of survival. To analyze the mechanisms of the systemic antitumor effect generated in this system, we assessed the cytolytic activity against inoculated tumors. The cytolytic activity of effector cells from treated animals was shown to be tumor specific, and was mainly CD8 and MHC class I (p<0.01) restricted. CD4 and MHC class-II treatment marginally inhibited the cytolytic activity but not significantly (p=0.07,0.08 respectively). The cytolysis of effector cells was more significantly enhanced by the treatment of both DC and chemotherapy, than that of either DC or chemotherapy alone. This study suggests that the strategy of i.t.injection of DC following low-dose chemotherapy could be a powerful weapon to treat patients with cancer in the clinical settings.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (17 results)

All 2004 Other

All Journal Article (13 results) Publications (4 results)

  • [Journal Article] Expression of cancer-testis antigen (CTA) genes in intrahepatic cholangiocarcinoma2004

    • Author(s)
      Utsunomiya T, Inoue H, Tanaka F et al.
    • Journal Title

      Ann Surg Oncol 11

      Pages: 934-940

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The high expression of Fractalkine results in a better prognosis for colorectal cancer patients2004

    • Author(s)
      Ohta M, Tanaka F, et al.
    • Journal Title

      Int J Oncol 26

      Pages: 41-47

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Identification of HLA-A24-restricted cytotoxic T lymphocytes epitope from cancer-testis antigen, NY-ESO-1, and induction of a specific antitumor immune response2004

    • Author(s)
      Yamaguchi H, Tanaka F, et al.
    • Journal Title

      Clin Cancer Res 10

      Pages: 890-896

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Clinical significance of Caveolin-1, Caveolin-2 and HER2/neu mRNA expression in human breast cancer2004

    • Author(s)
      Sagara Y, Mimori K, Tanaka F et al.
    • Journal Title

      Br J Cancer 91

      Pages: 959-965

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] A gene-expression signature can quantify the degree of hepatic fibrosis in the rat2004

    • Author(s)
      Utsunomiya T, Okamoto M, Tanaka F et al.
    • Journal Title

      J Hepatol 41

      Pages: 399-406

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma2004

    • Author(s)
      Ogawa K, Utsunomiya T, Tanaka F et al.
    • Journal Title

      Br J Cancer 91

      Pages: 282-286

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of Cancer-Testis Antigen(CTA) Genes in Intrahepatic2004

    • Author(s)
      Utsunomiya T, Inoue H, Tanaka F, et al.
    • Journal Title

      Ann Surg Oncol. 11

      Pages: 934-940

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of HLA-A24-restricted cytotoxic T lymphocytes epitope from cancer-testis antigen, NY-ESO-1,and induction of a specific antitumor immune response,2004

    • Author(s)
      Yamaguchi H, Tanaka F, et al.
    • Journal Title

      Clin Cancer Res 10

      Pages: 890-896

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical significance of Caveolin-1,Caveolin-2 and HER2/neu mRNA expression in human breast cancer2004

    • Author(s)
      Sagara Y, Mimori K, Yoshinaga K, Tanaka F, et al.
    • Journal Title

      Br J Cancer 91

      Pages: 959-965

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A gene-expression signature can quantify the degree of hepatic fibrosis in the rat2004

    • Author(s)
      Utsunomiya T, Okamoto M, Tanaka F, et al.
    • Journal Title

      J Hepatol 41

      Pages: 399-406

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma2004

    • Author(s)
      Ogawa K, Utsunomiya T, Tanaka F, et al.
    • Journal Title

      British J Cancer 91

      Pages: 282-286

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of cancer-testis antigen(CTA) genes in intrahepatic cholan giocarcinoma2004

    • Author(s)
      Utsunomiya T, Inoue H, Tanaka F, et al.
    • Journal Title

      Ann Surg Oncol 11

      Pages: 934-940

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Clinical significance of elongation factor-1 delt mRNA expression in oesophageal carcinoma2004

    • Author(s)
      Ogawa K, Utsunomiya T, Tanaka F et al.
    • Journal Title

      Br J Cancer 91

      Pages: 282-286

    • Related Report
      2004 Annual Research Report
  • [Publications] Yamaguchi H, Tanaka F et al.: "Identification of HLA-A24-restricted cytotoxic T lymphocytes epitope from cancer-testis antigen, NY-ESO-1, and induction of a specific antitumor immune response"Clin Cancer Res. 10. 890-896 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka F, Sonoda H et al.: "In vivo and in vitro inhibition of human cancer cell growth using adnoviral vector expressing FHIT gene : mechanism analysis using microarray"Mol Ther. 7. 418-419 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshinaga K, Inoue H, Tanaka F et al.: "Platelet-derived endothelial cell growth factor mediates rho-associated coiled-coil domain kinase messenger RNA expression and promotes cell motility"Ann Surg Oncol. 10. 582-587 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hashimoto W, Tanaka F et al.: "NK but not NKT cells play a necessary role to promote an innate antitumor response induced by IL-18"Int J Cancer. 103. 508-513 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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