| Project/Area Number |
15591523
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | National University Corporation Tottori University |
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Principal Investigator |
KAMITANI Hideki National University Corporation Tottori University, Faculty of Medicine, Associated Professor, 医学部, 助教授 (70194967)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Takashi National University Corporation Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (00175100)
TABUCHI Sadaharu National University Corporation Tottori University, Faculty of Medicine, Research Associate, 医学部, 助手 (40314603)
谷浦 晴二郎 鳥取大学, 医学部附属病院, 助手 (50291464)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | midbrain glioma / experimental brain tumor / C6 glioma / stereotactic implantation / 実験脳腫瘍 / C6グリオーマ / 局所注入 / 抗腫瘍効果 |
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| Research Abstract |
As the treatment of human intrinsic brainstem gliomas remains challenging, experimental glioma models are needed. We developed a rat model of intrinsic brain stem glioma that uses a stereotactic frame to fix the head for the delivery of C6 glioma cells to target sites via a permanently implanted cannula. We inoculated the rat midbrain, pons, or cerebral cortex with 5 x 10^4 cells suspended in 1 μl of culture medium over the course of 2 min. Three days post-implantation, tumor formation was visible in the periaqueductal gray matter in the midbrain and in the tegmentum of the pons. On the 10^<th> day, the tumor diameter exceeded over 2 mm ; length, there was no tumor-cell seeding into the cerebrospinal fluid space. The tumor manifested the histological features typical of glioblastoma ; Ki-67 labeling index was 32%. Because in our model the cannula is permanently implanted, additional inocula can be delivered. Finally, we establish the reproductive glioma model at the brain stem including midbrain with stereotactically accuracy.
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