Analysis of distribution of dihydrotestosterone concentration in prostate cancer tissue and differences of dihydrotestosterone and androgen induced gene expression after androgen deprivation

• Project/Area Number: 15591683
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kyoto University
• Principal Investigator: NISHIYAMA Hiroyuki (2004) Kyoto University, Urology, Lecturer, 医学研究科, 講師 (20324642); 木下 秀文 (2003) 京都大学, 医学研究科, 講師 (30324635)
• Co-Investigator(Kenkyū-buntansha): OGAWA Osamu Kyoto University, Urology, Professor, 医学研究科, 教授 (90260611) ; KAMOTO Toshiyuki Kyoto University, Urology, Associate Professor, 医学研究科, 助教授 (00281098) ; KINOSHITA Hidefumi Kansai Medical University, Urology, Associate Professor, 医学部, 助教授 (30324635) ; SEGAWA Takehiko Kyoto University, Urology, Assistant Professor, 医学研究科, 助手 (40343230) ; 山本 新吾 兵庫医科大学, 医学研究科, 講師 (80322741) ; 羽渕 友則 秋田大学, 医学研究科, 教授 (00293861) ; 西山 博之 京都大学, 医学研究科, 助手 (20324642)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: Prostate Cancer / Dyhydrotestosterone / Androgen receptor / アンドロゲン / 予後 / PSA / DHT / 内分泌療法 / アンドロゲン除去

Research Abstract

Objectives : Molecular studies suggest that ethnicity influences prostate tumor biology. We evaluated multiple known prognostic markers in localized prostate cancers using tissue microarrays (TMA) in Japanese patients.
Methods : Specimens were from 52 patients undergoing radical surgery at our institution between 1997 and 2001 without neoadjuvant hormonal therapy with complete 3 or more available cancer spots. Ki67, p53, and androgen receptor(AR) antigen expression were examined. Immunohistochemical scores were compared with outcomes of chemical relapse as monitored using prostate-specific antigen(PSA).
Results : Pathologic tumor (T) classification (p=0.047), World Health Organization(WHO) score (p=0.026), WHO histologic grade (p=0.026), and surgical margin status (p=0.018) were significant conventional clinicopathologic variables for predicting biochemical failure. TMA Gleason sum (p=0.038), TMA primary Gleason grade (p=0.013), Ki67 labeling index (LI) (p<0.0001), p53 (p=0.0097), and AR (p=0.0113) antigen expression also were significant. Moreover, surgical margin status and Ki67 LI were independently associated with treatment failure.
Conclusions : Especially together, Ki67 LI and p53 and AR expression in a localized prostate cancer often predicted postoperative progression in Japanese patients.

Research Products

• [Journal Article] Androgen receptor, Ki67, and p53 expression in radical prostatectomy specimens predict treatment failure in Japanese population.
  • Author(s): 井上貴博, 清川岳彦, 木下秀文, 他
  • Journal Title: Urology (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Androgen receptor, Ki67, and p53 expression in radical prostatectomy specimens predict treatment failure in Japanese population.
  • Author(s): Takahiro Inoue, Takehiko Segawa, Taizou Shiraishi, Toru Yoshida, Yoshinobu Toda, Tomoni Yamada, Naoko Kinukawa, Hidefumi Kinoshita, Toshiyuki Kamoto, Osamu Ogawa
  • Journal Title: Urology (in press)
  • Description: 「研究成果報告書概要(欧文)」より