The production and regulation of angiogenic factor or anti-angiogenic factor by ovary and uterine endometrial tissues.
Grant-in-Aid for Scientific Research (C)
|Allocation Type||Single-year Grants|
Obstetrics and gynecology
|Research Institution||Oita University(2004-2006)|
KAWANO Yasushi Oita Univ., Dept. of Obstet. & Gynecol., Assist Professor, 医学部, 講師 (40274758)
NARAHARA Hisashi Oita Univ., Dept. of Obstet. & Gynecol., Professor, 医学部, 教授 (60211447)
FUKUDA Junichiro Oita Univ., Dept. of Obstet. & Gynecol., Instructor, 医学部, 助手 (20381048)
宮川 勇生 大分大学, 医学部, 教授 (70040607)
嶺 真一郎 大分大学, 医学部, 助手 (00336273)
|Project Period (FY)
2003 – 2006
Completed(Fiscal Year 2006)
|Budget Amount *help
¥3,600,000 (Direct Cost : ¥3,600,000)
Fiscal Year 2006 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 2005 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 2004 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 2003 : ¥2,100,000 (Direct Cost : ¥2,100,000)
|Keywords||Vascular endothelial growth factor / Endometrial stromal cells / Follicular fluid / 顆粒膜細胞 / 子宮内膜 / 血管新生|
Our objective was to clarify the physiological role of angiogenic factor or anti-angiogenic factor by ovarian granulosa cell, endometrial stromal cells (ESC) and amnion-derived (WISH) cells.
Several angiogenic factors were present in human follicular fluid. macrophage colony-stimulating factor, monocyte chemoattractait protein-1 and macrophage inflammatory protein-3 a were secreted by granulosa cells. These factors were up-regulared by interleukin-1 a and tumor necrosis factor-a by these cells.
We reported that VEGF was produced by ESC. In adition thrombospondin (TSP)-1 production was increased by interferon (IFN)-γ and was decreased by epidermal growth factor (EGF) in a dose-dependent manner. TSP-1 production and TSP-1 mRNA expression were also increased by medroxyprogesterone (MPA) and cyclic AMP during in vitro decidualization..
WISH cells were cultured, and VEGF production was increased by EGF or insulin-like growth factor (IGF)-1 in a dose-dependent manner. The activation of MAP kinase and akt, which is phosphorylated by PI 3-kinase, were detected by western blot analysis. VEGF production was significantly decreased following pretreatment with MEK inhibitor (U0126) or PI 3-kinase inhibitor (wortmannin), and then treated with EGF or IGF-1.
It is suggest ed that angiogenic factor may contribute to the neovascularization and proliferation of the ovary, uterine endometrium, and placenta.
Research Products (17results)