Immunological analysis of dendritic cell-osteopontin system in the remodeling of serious ocular allergy
| Project/Area Number |
15591869
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Fukuoka University (2005)
Yokohama City University (2003-2004) |
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Principal Investigator |
UCHIO Eiichi Fukuoka University, School of Medicine, Professor, 医学部, 教授 (70232840)
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| Co-Investigator(Kenkyū-buntansha) |
西出 忠之 横浜市立大学, 医学部, 助手 (90347344)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Vernal keratoconjunctivitis / Langerhans cell / Dendritic cell / Osteopontin / Atopic dematitis / Interleukin-4 / アレルギー性結膜炎 / マウス / カドヘリン / インテグリン / 結膜 / アレルギー / ステロイド / マスト細胞 / 好酸球 / T細胞 / 低親和性IgE受容体 |
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| Research Abstract |
The frequency of IL-4-producing conjunctival T cells in patients with vernal keratoconjunctivitis (VKC) and atopic keratoconjunctivitis (VKC) were significantly higher than that in patients with allergic conjunctivitis (AC). The frequencies of IL-4-producing conjunctival CD4+ T cells of the patients with AC, AKC, or VKC were significantly higher than those of peripheral blood CD4+ T cells of the same disease. Further, increased frequency of P-selectin ligand expressing IL-4-producing T cells was observed in conjunctiva compared to that in PBMC of the patients with allergic conjunctival diseases. The frequencies of IL-4-producing peripheral blood T cells in the patients with ACD were sig-nificantly higher than that in normal controls. Tear levels of cytokines, interleukin-2(IL-2), interferon gamma (IFN-γ), IL-4, IL-5, IL-6, IL-8 and IL-13, in 49 patients with ACD (allergic conjunctivitis 14 cases, atopic keratoconjunctivitis 14 cases and vernal keratoconjunctivitis 21 cases) were measured with ELISA (enzyme-linked immunosorbent assay) method. Correlation between cytokines were statistically analyzed. Significant correlations were observed between IFN-g and IL-13, IL-4 and IL-13, IL-5 and IL-6, and IL-8 and IL-13. These results suggest that IL-4-producing T cells that have infiltrated into the conjunctiva play an important role in the immunopathogenesis of allergic conjunctival diseases, and P-selectin ligand is involved in the entry of T cells into allergic inflammatory site and correlation between tear cytokines show various patterns suggesting the complexity and redundancy of cytokines.
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Report
(4 results)
Research Products
(28 results)
