| Project/Area Number |
15591908
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
KUMAGAI Norio St.Marianna University School of Medicine, Professor, 医学部, 教授 (30103477)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Hajime St.Marianna University School of Medicine, Lecturer, 医学部, 講師 (60193603)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | cultured keratinocytes / skin regeneration / fibrin scaffold / regenerative medicine / cell therapy / adult stem cells / 線維芽細胞 / フィブリン / 血管新生 |
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| Research Abstract |
Cultured epithelium and cultured dermis in particular have considerably preceded regeneration of other organs in the field of regenerative medicine. Some reasons why regeneration of epithelial tissue has preceded regeneration of other tissues seem to be that epithelial tissue has the structural advantages of being two-dimensional and needing no rigid scaffold as well as being anatomically simple as compared to other organs and tissues. With regard to keratinocytes, somatic (adult) stem cells have been found to be present in hair roots. Another reason is that it is relatively easy to harvest the stem cells. We have already transplanted cultured keratinocytes to treat large skin defects in at least 500 patients. The regenerated epithelium must be separated from the dish base for grafting. It is usually separated from the dish base with a proteinase, and for grafting the separated sheet is placed on a carrier prepared by molding in the same shape as the dish. There would be no problem if the epithelium sheet were strong enough to pick up with tweezers, but it is impossible to pick up the separated regenerated epidermis sheet with tweezers directly, and a carrier is required for application of the separated regenerated epithelium from the dish to the skin graft bed in clinical practice. Furthermore, because regenerated epithelium lacks a dermal component, epithelium grafting alone cannot produce good results in areas where there is a dermal defect. The facts mean that dermal elements are required for take of epithelium graft. To solve these problems, we have developed a technique designed to avoid the influence of protease released by keratinocytes and to increase the physical strength of fibrin itself, by mixing a certain protein with fibrin scaffold (Patent pending).
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