| Project/Area Number |
15592098
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Saitama Medical School |
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Principal Investigator |
YODA Tetsuya Saitama Medical School, Medicine, Professor, 医学部, 教授 (60242210)
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| Co-Investigator(Kenkyū-buntansha) |
TOMARU Yasuhisa Saitama Medical School, Medicine, Assistant Professor, 医学部, 講師 (50348207)
SHIMAMURA Yumiko Saitama Medical School, Medicine, Instructor, 医学部, 助手 (10348287)
HOSHI Kazuto Univ. of Tokyo Hospital, Associate Professor, 医学部附属病院, 寄付講座教員 (30344451)
OGASAWARA Toru Univ. of Tokyo Hospital, Instructor, 医学部附属病院, 寄付講座教員 (20359623)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | cartilage regeneration / humoral factor / dedifferentiation / temporomandibular joint / statistical analysis / Cdk6 / CGK II / cell differentiation / CGK II / 軟骨 / 三次元培養 / 再生医療 |
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| Research Abstract |
Regenerative medicine of the temporomandibular joint (TMJ) is one of the most important subjects in the field of oral and maxillofacial surgery. In order to provide a basis of therapeutic application of TMJ cartilage regeneration, we have investigated in the following projects.
(1)Basic study to prepare a chondrocyte proliferation medium : In this project, we used cartilage cells derived from the cartilage of the nasal septum and the remnant auricular cartilage. We have aimed to prepare a chondrocyte proliferation medium that i)does not contain fetal bovine serum (FBS), ii)provides more than a 1000-fold increase in cell numbers, and iii)makes use of a combination of commercially available growth factors that has been proven to be effective for clinical use. As a result, a combination of FGF-2, insulin, and IGF- 1 synergistically enhanced the proliferation. Furthermore, we showed that a combination of BMP-2, insulin, and PTH possessed promotional effects on proliferation of hypertrophic
… More
differentiation of chondrocyte. Finally, we evaluated the property of the scaffold using some kinds of hydro-gel on cartilage regeneration.
(2)Understanding the molecular mechanism of chondrocyte differentiation : We have investigated a novel role of CGK II in hypertrophic differentiation of chondrocytes and a critical function of Cdk 6 as a negative regulator of differentiation of osteoblast, osteoclast and chonrocyte. As a result, CGK II, a molecular switch, coupled the cessation of proliferation and the start of hypertrophic differentiation of chondrocytes through attenuation of Sox 9 function. Cdk 6 was a critical regulator of BMP-2-induced osteoblast differentiation by Smads-mediated down-regulation and, RAW cells overexpressing Cdk 6 resisted RANKL-induced osteoclastgenesis ; however, cell cycle regulation was not affected by the levels of Cdk 6 overexpression. In conclusion, we have demonstrated in vitro evidence of the importance of these molecules in proliferation and differentiation of bone tissue. Less
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