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Elucidation of the regulatory mechanism of calprotectin expression in oral epithelial cells and basic study of application for periodontal therapy

Research Project

Project/Area Number 15592190
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionThe University of Tokushima

Principal Investigator

ASANO Masahiro (2004-2005)  The University of Tokushima, Medical・Dental Hospital, Research assistant, 医学部・歯学部附属病院, 助手 (30335822)

木戸 淳一 (2003)  徳島大学, 歯学部, 助教授 (10195315)

Co-Investigator(Kenkyū-buntansha) KATAOKA Masatoshi  The University of Tokushima, Institute for Genome Research, Associate Professor, ゲノム機能研究センター, 助教授 (20224438)
KIDO Jun-ichi  The University of Tokushima, Institute of Health Biosciences, Associate Professor, 大学院・ヘルスバイオサイエンス研究部, 助教授 (10195315)
浅野 将宏  徳島大学, 医学部・歯学附属病院, 助手 (30335822)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsCalprotectin / Antimicrobial peptide / Epithelial cells / Differentiation regulatory factors / Periodontal diseases / Immunotherapy / 分化 / 口腔上皮細胞
Research Abstract

Calprotectin (S100A8/S100A9) is an antimicrobial peptide which is produced by epithelial cells, and inhibits the growth of periodontophatic bacteria and adhesion of bacteria to epithelium. Calprotectin plays an important role in periodontal immunity, but the regulatory mechanism of calprotectin expression is not well known. In the present study, I investigated the factors which regulated calprotectin expression in human gingival keratinocytes and its regulatory mechanism. IL-1α and calcium, the up-regulators of keratinocyte differentiation, significantly increased mRNA expression of S100A8/S100A9 and calprotectin protein. On the other hand, TGF-β, the down-regulator of differentiation, clearly decreased the expression of calprotectin genes and protein. Furthermore, retinoic acid, the down-regulator of differentiation, suppressed the IL-1α-induced calprotectin expression. I prepared the deletion constructs containing promoter region of S100A8 or S100A9 gene and investigated the mechanism by luciferase assay. I found the decrease of luciferase activity in -765/-722 and -256/-111 regions of S100A8 deletion constructs, and in -188/-53 region of S100A9 deletion constructs. These region of two genes contained the DNA binding site to C/EBP, transcriptional factor. I showed the increase of C/EBP DNA binding activity in gingival keratinocytes stimulated by calcium and Il-1α. Taken together, calprotectin expression in gingival keratinocytes is regulated by the factors which affect keratinocyte differentiation and their regulation is related to C/EBP. I think that the elucidation of calprotectin expression in gingival keratinocytes plays a role in the development of periodontal immunotherapy using antimicrobial peptides.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (15 results)

All 2005 2004 2003 Other

All Journal Article (15 results)

  • [Journal Article] Calprotectin expression in human monocytes : Induction by Porhyromonas gingivalis lipopolysaccharide, tumor necrosis factor-α, and interleukin lβ.2005

    • Author(s)
      Suryono
    • Journal Title

      J Periodontol 76

      Pages: 437-442

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Calprotectin expression in human monocytes : Induction by Porhyromonas gingivalis lipopolysaccha-ride, tumor necrosis factor-α, and interleukin 1β.2005

    • Author(s)
      Suryono
    • Journal Title

      J Periodontol 76

      Pages: 437-442

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Calprotectin expression in human monocytes : Induction by Porhyromonas gingivalis lipopolysaccharide, tumor necrosis factor-α, and interleukin 1β.2005

    • Author(s)
      Suryono
    • Journal Title

      J Periodontol 76

      Pages: 437-442

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Induction of calprotectin release by Porhyromonas gingivalis lipopolysaccharide in human netrophils.2004

    • Author(s)
      Jun-ichi Kido
    • Journal Title

      Oral Microbiology and Immunity 19

      Pages: 182-187

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Induction of calprotectin release by Porphyromonas gingivalis lipopolysaccharide in human neutrophils.2004

    • Author(s)
      Jun-ichi Kido
    • Journal Title

      Oral Microbiology and Immunology Vol.19 No.3

      Pages: 182-187

    • Related Report
      2004 Annual Research Report
  • [Journal Article] カルプロテクチンおよび1型プロコラーゲンペプチドの歯周病診断指標としての有用性2003

    • Author(s)
      木戸淳一
    • Journal Title

      日本歯周病学会会誌 45巻秋季号

      Pages: 52-52

    • Related Report
      2004 Annual Research Report
  • [Journal Article] IL-1αおよびTGF-βがヒト歯肉上皮細胞のカルプロテクチン発現に及ぼす影響2003

    • Author(s)
      林 能理子
    • Journal Title

      日本歯周病学会会誌 45巻秋季号

      Pages: 143-143

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Calprotectin release from human neutrophils is induced by Porphyromanas gingivalis lipopolysaccharides via the CD-14-Toll-like receptor-nuclear factor κB pathway.2003

    • Author(s)
      Jun-ichi Kido
    • Journal Title

      Journal of Periodontal Research Vol.38 No.6

      Pages: 557-563

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effect of Porphyromanas gingivalis lipopolysaccharide, tumor necrosis factor-α, and interleukin-1β on calprotectin release in human monocytes.2003

    • Author(s)
      Suryono
    • Journal Title

      Journal of Periodontology Vol.74 No.12

      Pages: 1719-1724

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Regulation of calprotectin expression by IL-lα and TGF-β in human gingival keratinocytes.

    • Author(s)
      Noriko Hayashi
    • Journal Title

      J Periodont Res (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Norepinephrine stimulates calprotectin expression in human monocytic cells.

    • Author(s)
      Suryono
    • Journal Title

      J Periodont Res (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regulation of calprotectin expression by IL-1α and TGF-β in human gingival keratinocytes.

    • Author(s)
      Noriko Hayashi
    • Journal Title

      J Periodont Res (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Norepinephrine stimulates calprotectin expression in human monocytic cells.

    • Author(s)
      Suryono
    • Journal Title

      J Periodont Res (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regulation of calprotectin expression by IL-1α and TGF-β in human gingival keratinocytes.

    • Author(s)
      Noriko Hayashi
    • Journal Title

      J Periodont Res (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Norepinephrine stimulates calprotectin expression in human monocytic cells.

    • Author(s)
      Suryono
    • Journal Title

      J Periodont Res (In press)

    • Related Report
      2005 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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