Mapping of Nuclear Matrix Binding Region of Human Genome and Functional Analysis of A Nuclear Matrix Protein
| Project/Area Number |
15602002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
ポストゲノムのナノサイエンス
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| Research Institution | JOSAI UNIVERSITY |
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Principal Investigator |
HIBINO Yasuhide Josai University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (10189805)
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| Co-Investigator(Kenkyū-buntansha) |
OKAZAKI Mari Josai University, Faculty of Pharmaceutical Sciences, Lecturer, 薬学部, 講師 (50272901)
USUI Tatsuhiro Josai University, Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (40337481)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Human Genome / Nuclear Matrix / DNA Binding Protein / Gene Expression / MAR / SAR / Bioinfomatics / Transcription Factor / 核基質 / 転写活性 / matrin3 / クロマチン免疫沈降 / matrin 3 |
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| Research Abstract |
We examined the molecular basis of rat P130, a nuclear scaffold protein, and its functions. P130 comprising 845 amino acid residues possesses several functional domains and yields an electrophoretically distinctive isoform, P123, by altering its phosphorylation status in association with translocation across the nuclear membrane and from the digitonin-extractable fraction of the nucleus to the nuclear scaffold. The functional domains, NLS, NES, and zinc-finger bearing DNA-binding domains, ZF1 and ZF2, aid these translocations. P130 binds RNA through two RNA-binding domains (RB1 and RB2) similar to those of hnRNPs I and L.Microsome- and polysome-localized P130 and P123 were found in rat liver and Ac2F hepatoma cells. This localization required prior entry of P130 to the nucleus, but did not require RB1 and RB2. Thus, P130 initially purified from rat liver nuclear scaffold has the potential to play a variety of roles in biological events not only in the nuclear scaffold but also in various subcellular compartments. P130 (AB205483) is identical to matrin 3 (M63485 and BC062231), although the primary structure of rat matrin 3 has been revised, since it was first published.
The Human Genome Database (GDB) (Future et al. 2000 ; http::/gdbwww.gdb.org/) was used for MAR/SAR search program [MARFinder or SMARTest (Friscm et al. 2002 ; http://www.genomatix.de/cgi-bin/smartest pd/smartest.pl)] As a consequence, we have mapped more than 100 MARs/SARs of 30 genes in human genome.
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Report
(4 results)
Research Products
(11 results)
