Development of chemical sensor arrays for detecting of oligopeptides.
| Project/Area Number |
15608002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
コンビナトリアル科学
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
HIOKI Hideaki Tokushima Bunri University, Faculty of Pharmaceutical Sciences, Associate professor, 薬学部, 助教授 (00208737)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Calix[4]arenes / Chemical sensors / Microarray / マイクロアレイ |
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| Research Abstract |
Chemical Sensors are of increasing importance as convenient analytical method without pretreatment. In spite of a large number of metal ion sensors have been developed, sensors for organic molecules are still very limited. In this research I attempted to develop sensor arrays for multicomponent organic molecules. First, I investigated the conditions to immobilize the organic substrate on a slide glass. After extensive screening, I succeeded to attach the fluorophores on a glass slides which modified aminopropylsilyl group. Unfortunately, some fluorophores on the glass were unstable to the fluorescence excitation. We have explored stable fluorophores suitable for sensors on glasses. As a result, the compound possessing dansyl group as a fluorophore was very stable.
Next we tried to synthesize the sodium ion sensor based on a calix[4]arene in 13 steps. The compound possesses aminoethylated acetamide group as a sodium ion binding site, dancyl group as a fluorophore and nitrophenyl group as a quencher. Unexpectedly it did not respond to sodium ion in the liquid phase. The pair of dancyl - nitorphenyl may not suitable pair for the sensing. The effective pairs for sensing are currently under investigation.
We also investigated to synthesize the calix[4]arene library substituted at the upper rim. Some peptidocalix[4]arenas in the library selectively bind the oligopeptide, which have acted as chemical sensors for oligopeptides in liquid phase. Immobilization of them on a slide glass is also under investigation.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Enantioselective total synthesis of Eurylene, 14-Deacetyl eurylene, and their 11-epimers : The relation between ionophoric nature and cytotoxicity.2004
Author(s)
Hideaki Hioki, Suzuyo Yoshio, Masatoshi Motosue, Yuka Oshita, Yukari Nakamura, Daisaku Mishima, Yoshiyasu Fukuyama, Mitsuaki Kodama, Keisuke Ueda, Takashi Katsu
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Journal Title
Org.Lett. 6, No.6
Pages: 961-964
Description
「研究成果報告書概要(欧文)」より
Related Report
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