| Budget Amount *help |
¥39,130,000 (Direct Cost: ¥30,100,000、Indirect Cost: ¥9,030,000)
Fiscal Year 2018: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2017: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2016: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2015: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
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| Outline of Final Research Achievements |
A novel method of cell fusion is developed that allows the 1:1 fusion among two types of cells with 80-90% yield. The method uses a micro-fluidic device with an array of orifice between two flow channels, and when the voltage is applied between the channels, filed constrictions are created at the orifice, by which the cells are attracted to the orifice to form cell pair. The method enables the mixing of gene / cytoplasmic factors / membrane channels between the fused cells. Using the method, a real-time observation is made on the initialization of a somatic cell fused with an ES cell. Based on the result, a microdevice is developed for the initialization utilizing iPS cell, having more availability compared with ES, where the cytoplasm of the somatic cell is replaced with that of the iPS cell through repeated fusion and separation.
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