Creation of new genome science using chemical tools to precisely detect modification and damage of human genome
| Project/Area Number |
15H02189
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | National Institute for Materials Science |
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Principal Investigator |
Komiyama Makoto 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, NIMS招聘研究員 (50133096)
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| Co-Investigator(Kenkyū-buntansha) |
須磨岡 淳 東京工科大学, 工学部, 教授 (10280934)
徐 岩 宮崎大学, 医学部, 教授 (40506763)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIGI Narumi 物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, NIMS特別研究員 (00396780)
Tanaka Makiko 電気通信大学, 大学院情報理工学研究科, 助教 (90397703)
Rajendran Arivazhagan 京都大学, エネルギー理工学研究所, 講師 (90723122)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥43,290,000 (Direct Cost: ¥33,300,000、Indirect Cost: ¥9,990,000)
Fiscal Year 2017: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2016: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2015: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
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| Keywords | ゲノム / PNA / インベージョン / ゲノム断片分離 / エピゲノム / DNA結合タンパク質 / DNAメチル化 / テロメア / DNA損傷 |
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| Outline of Final Research Achievements |
We already developed super artificial restriction enzyme. In this research, we added affinity separating ability to this tool, and constructed the second- generation super artificial restriction enzyme. By modifying biotin to PNA which is a constituent of super restriction enzyme, we successfully extracted desired DNA fragments of several thousand base pairs in high purity and high yield from the human genome with 3 billion base pairs. Furthermore, by combining this biotin-modified PNA with S1 nuclease, we also succeeded in extracting desired DNA fragments from the human genome in one pot.
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Report
(4 results)
Research Products
(19 results)
