Comprehensive analysis of the molecular mechanisms of microRNA action
| Project/Area Number |
15H02382
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Tomari Yukihide 東京大学, 分子細胞生物学研究所, 教授 (90447368)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥40,300,000 (Direct Cost: ¥31,000,000、Indirect Cost: ¥9,300,000)
Fiscal Year 2017: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2016: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2015: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
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| Keywords | microRNA / 翻訳抑制 / poly(A)分解 / Argonaute / mRNA分解 |
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| Outline of Final Research Achievements |
We conducted our research by focusing on how microRNAs mediate gene silencing of their target mRNAs by utilizing two distinct molecular mechanisms: "translational repression" and "poly(A) shortening". We identified a novel E3 ubiquitin ligase named "Iruka", which acts to ensure the quality of Argonaute proteins, the core factor in the microRNA pathway. Moreover, we revealed a series of new insights into the enzymatic properties and their ATP dependence of the deadenylase enzymes in the CCR4-NOT complex, which is recruited by microRNAs to mediate the poly(A) shortening of their target mRNAs.
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Report
(4 results)
Research Products
(7 results)
