| Budget Amount *help |
¥42,120,000 (Direct Cost: ¥32,400,000、Indirect Cost: ¥9,720,000)
Fiscal Year 2018: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2017: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2015: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
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| Outline of Final Research Achievements |
We found a novel type of glycoside hydrolase (beta-L-Araf-ase HypBA1) that has the Cys nucleophile coordinated to Zn. In this study, an examination of its reaction mechanism and structural analysis of related enzymes (proteins) were performed. Novel mechanism-based inhibitors that specifically react with the Cys residue were developed. Using these inhibitors, we could reveal the three-dimensional structures of all of the reaction steps of HypBA1. We also found novel beta-L-Araf-ases and performed functional and structural analyses with related enzymes. Characteristics of the enzymes and proteins involved in the beta-arabinooligosaccharide-degradation were revealed.
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