Development of new breeding technology for marine fish using full-life cycle aquaculture system and genome editing
| Project/Area Number |
15H02459
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
北野 載 九州大学, 農学研究院, 助教 (30635008)
坂口 圭史 九州大学, 農学研究院, 准教授 (50396280)
長野 直樹 宮崎大学, 農学部, 教授 (50437943)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥42,250,000 (Direct Cost: ¥32,500,000、Indirect Cost: ¥9,750,000)
Fiscal Year 2018: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2017: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2016: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2015: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
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| Keywords | ゲノム編集 / 育種 / マサバ / 完全養殖 / 攻撃性 / 生殖 / TALEN / カタクチイワシ / CRISPR/Cas9 / CRISPR/Cあｓ9 / 受精卵 / 水産学 |
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| Outline of Final Research Achievements |
Although chub mackerel is a promising target species for aquaculture, its survival rate is remarkably low due to co-eating during the juvenile season, like bluefin tuna. In this study, we aimed to create a strain of chub mackerel that destroyed AVTR-V1a2, an aggression-related gene, using genome editing technology. As a result, we succeeded in producing 21 individuals in which both alleles of AVTR-V1a2 were destroyed by TALEN (homo KO). In the future, we plan to use these individuals to produce large quantities of fry and evaluate their characters.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、完全養殖系のマサバを対象として、攻撃性に関わる遺伝子をゲノム編集技術により破壊することで、共喰い行動を抑え生残率の向上した、養殖により適した形質をもつマサバの品種作製を試みたものである。本研究により、海産魚を対象にしたゲノム編集
を利用した育種の基盤技術が整備された。ゲノム編集食品の流通が解禁された現在、今後、成長産業として有望視されている魚類養殖産業において、育種の加速化が進展することが期待される。
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Report
(5 results)
Research Products
(13 results)
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[Presentation] Production of AVTR-V1a2 knockout strain in chub mackerel, Scomber japonicus, by genome editing with TALEN.2019
Author(s)
Sakanoue R, Ohga H, Shibata K, Nagano N, Kitano H, Sakaguchi K, Kuhara S, Tashiro K, Kim S, Nagasako T, Uchida S, Sakuma , Yamamoto T, Gen K, Fujiwara A, Kazeto Y, Kobayashi T, Ohta K, Matsuyama M
Organizer
Marine Biotechnology Conference 2019 in Shizuoka
Related Report
Int'l Joint Research
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[Presentation] F0 FOUNDER PRODUCTION OF AVTR-V1A2 KNOCKOUT STRAIN2018
Author(s)
Ohga H, Shibata K, Kai S, Sakanoue R, Kitano H, Ohta K, Nagano n, Sakaguchi K, Kuhara S, Tashiro K, Kim SW, Sakuma T, Yamamoto T, Gen K, Fujiwara A, Kazeto Y, Kobayashi T, Matsuyama M
Organizer
Aquaculture America 2018
Related Report
Int'l Joint Research
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[Presentation] Development of genome editing technology in chub mackerel, Scomber japonicus and knockout of aVTR-V1a2 by TALEN2017
Author(s)
Shibata K, Ohga H, Nagano N, Sakaguchi K, Kitano H, Ohta K, Kuhara S, Tashiro K, Kim S, Sakuma T, Yamamoto T, Gen , Fujiwara A, Kazeto Y, Kobayashi T, Matsuyama M
Organizer
International Symposium “Fisheries Science for the Future Generations”.
Related Report
Int'l Joint Research
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[Presentation] Gene knockout of AVTR-V1a2 by TALEN in chub mackerel, Scomber japonicas.2016
Author(s)
2.Shibata K, Ohga H, Nagano N, Sakaguchi K, Kitano H, Kuhara S, Tashiro K, Kim S, Sakuma T, Yamamoto T, Gen K, Fujiwara A, Kazeto Y, Kobayashi T, Matsuyama M.
Organizer
13th International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia
Place of Presentation
Daejeon, Korea
Year and Date
2016-11-09
Related Report
Int'l Joint Research
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