| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
We established LacO-I-SceI-TetO cell line and tried to set up a new system for monitoring chromosomal translocation, however unfortunately, it was not working well because of technical problems. It is well known that the particle irradiation more frequently causes chromosomal translocation compared with X-ray irradiation. Therefore we simultaneously visualized the site of IR-induced DSBs and chromosome position by combining Immunofluorescence and FISH after C-ion or X-rays irradiation. We found that the frequency of gH2AX foci at the chromosome boundary of chromosome 1 after C-ion irradiation was >4-fold higher than that after X-ray irradiation, and gH2AX foci at chromosome boundaries after C-ion irradiation contain DSBs undergoing DNA-end resection, which promotes repair utilizing microhomology mediated end-joining during translocation. These data suggest that the spatial proximity between breaks is an important factor in translocation formation.
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