Elucidation of molecular mechanism of transcription-coupled nucleotide excision repair
| Project/Area Number |
15H02820
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Osaka University |
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Principal Investigator |
SAIJO Masafumi 大阪大学, 生命機能研究科, 准教授 (90221986)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | ヌクレオチド除去修復 / コケイン症候群 / 紫外線高感受性症候群 / ユビキチン化 / SUMO化 / UV / 紫外線 / DNA修復 / ゲノム |
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| Outline of Final Research Achievements |
We analyzed transcription-coupled nucleotide excision repair (TC-NER), a subpathway of nucleotide excision repair that rapidly removes transcription-blocking DNA damage, and the following results on the functions of TC-NER factors were obtained. (1) The C-terminal region and SUMOylation of CSB plays critical roles in TC-NER. (2) UVSSA is degraded by proteasome upon dissociation from USP7, resulting in TC - NER deficiency, but when a mutation is introduced at the ubiquitination site, degradation is suppressed and TC - NER becomes normal. (3) Ubiquitination of RNA polymerase II may be involved in the restart of transcription after removal of transcription-blocking DNA damage. These results are important cues to reveal molecular mechanism of TC-NER.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] DGCR8 Mediates Repair of UV-Induced DNA Damage Independently of RNA Processing.2017
Author(s)
Philamer C. Calses, Kiranjit K. Dhillon, Nyka Tucker, Yong Chi, Jen-wei Huang, Masaoki Kawasumi, Paul Nghiem, Yemin Wang, Bruce E. Clurman, Celine Jacquemont, Philip R. Gafken, Kaoru Sugasawa, Masafumi Saijo, Toshiyasu Taniguchi
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Journal Title
Cell Reports
Volume: 19
Issue: 1
Pages: 162-174
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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