DEVELOPMENT OF MICROBIAL COMMUNITY DESIGN TECHNOLOGY USING THE RHIZOSPHERE OF AQUATIC PLANTS AND ITS APPLICATION TO ENVIRONMENTAL WATER QUALITY CONTROL

• Project/Area Number: 15H02845
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Modeling and technologies for environmental conservation and remediation
• Research Institution: Kitasato University
• Principal Investigator: SEI Kazunari 北里大学, 医療衛生学部, 教授 (80324177)
• Co-Investigator(Kenkyū-buntansha): 古川 隼士 北里大学, 医療衛生学部, 講師 (90632729) ; 井上 大介 北里大学, 医療衛生学部, 准教授 (70448091)
• Research Collaborator: SAWADA Kazuko
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: 水生植物根圏 / 遺伝子発現解析 / 微生物群集構造解析 / 水質浄化

Outline of Final Research Achievements

The aim of this study was to clarify the mechanism of aquatic plants to accumulate specific microorganisms in the rhizosphere from the characteristics of microbial communities formed in the rhizosphere and gene expression analysis of aquatic plants coexisting with rhizosphere microorganisms.
In the rhizosphere of Spirodela polyrrhiza and Lemna minor, two microorganism groups which show different accumulating behavior were confirmed. The one group showed quick, that is within 3 days, accumulation to the rhizosphere. Another group showed slow accumulation but steadily over time. As the characteristic of the microorganism group selectively accumulating in the rhizosphere, the presence of motility seemed one of important factors.
Although the gene expression analysis of S. polyrrhiza coexisting with rhizobacteria was failed because of the low quality of mRNA extracted from S. polyrrhiza, we could develop a series of experimental methods including improvement points.

Research Products

• [Journal Article] Draft genome sequence of Sphingobium fuliginis OMI, a bacterium that degrades alkylphenols and bisphenols (2017)
  • Author(s): Kuroda, M., Ogata, Y., Yahara, T., Yokoyama, T., Ishizawa, H., Takada, K., Inoue, D., Sei, K., Ike, M.
  • Journal Title: Genome Announcements Volume: 5 Issue: 47
  • DOI: 10.1128/genomea.01323-17
  • Peer Reviewed / Open Access
• [Presentation] 水生植物根圏に形成される微生物群集の特殊性 (2016)
  • Author(s): 清和成、澤田和子、井上大介、玉木秀幸、遠山忠、田中靖浩、森一博、黒田真史、池道彦、森川正章
  • Organizer: 第19回日本水環境学会シンポジウム
  • Place of Presentation: 秋田県立大学（秋田県秋田市）
  • Year and Date: 2016-09-14
  • Invited
• [Presentation] Analysis of microbial community enriched in the rhizosphere of Spirodela polyrrhiza and Lemna minor cultivated in river water (2016)
  • Author(s): Kazuko Sawada, Ryo Yamato, Daisuke Inoue, Masaaki Morikawa, Kazunari Sei
  • Organizer: Water and Environment Technology Conference 2016
  • Place of Presentation: 中央大学（東京都文京区）
  • Year and Date: 2016-08-28
  • Int'l Joint Research