| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The objective of this study is to develop a new technology that allows for high-throughput i.e. highly efficient visualization/quantification of cellular traction forces. Hydrophilic membrane is created in a spatially uniform manner over the surface of silicone materials. With cells plated on the flexible membrane, microscopy is performed to detect deformations of the membrane induced upon the loading of cellular contractile forces. The microscope is equipped with a stage incubator and stepping motors, with which time-lapse of the cells and membrane are imaged at multiple points of the membrane substrate. With image analyses on the acquired series of the images, the magnitude of the deformations of the membrane is quantified. Automatic image analysis code is built to compute the magnitude of the cellular contractility-driven deformations of the membrane. The system developed here is applicable to cell biology studies as a tool to quantify the contractility of individual cells.
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