Development of cell contraction assay
| Project/Area Number |
15H03004
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Osaka University (2016-2017)
Nagoya Institute of Technology (2015) |
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Principal Investigator |
Deguchi Shinji 大阪大学, 基礎工学研究科, 教授 (30379713)
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| Research Collaborator |
MATSUI Tsubasa 大阪大学, 大学院基礎工学研究科, 講師 (50638707)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 細胞バイオメカニクス / メカノバイオロジー / 細胞収縮力 / メカノミクス / 非筋II型ミオシン / トラクションフォースマイクロスコピー / バイオイメージング |
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| Outline of Final Research Achievements |
The objective of this study is to develop a new technology that allows for high-throughput i.e. highly efficient visualization/quantification of cellular traction forces. Hydrophilic membrane is created in a spatially uniform manner over the surface of silicone materials. With cells plated on the flexible membrane, microscopy is performed to detect deformations of the membrane induced upon the loading of cellular contractile forces. The microscope is equipped with a stage incubator and stepping motors, with which time-lapse of the cells and membrane are imaged at multiple points of the membrane substrate. With image analyses on the acquired series of the images, the magnitude of the deformations of the membrane is quantified. Automatic image analysis code is built to compute the magnitude of the cellular contractility-driven deformations of the membrane. The system developed here is applicable to cell biology studies as a tool to quantify the contractility of individual cells.
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Report
(4 results)
Research Products
(57 results)
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[Journal Article] Cellular force assay detects altered contractility caused by a nephritis-associated mutation in nonmuscle myosin IIA2017
Author(s)
Fukuda, S.P., Matsui, T.S., Ichikawa, T., Furukawa, T., Kioka, N., Fukushima, S., Deguchi, S.
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Journal Title
Development, Growth & Differentiation
Volume: 59
Issue: 5
Pages: 423-433
DOI
Related Report
Peer Reviewed
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[Journal Article] Vinexin family (SORBS) proteins play different roles in stiffness-sensing and contractile force generation2017
Author(s)
Ichikawa, T., Kita, M., Matsui, T.S., Ichikawa-Nagasato, A., Araki, T., Chiang, S.H., Sezaki, T., Kimura, Y., Ueda, K., Deguchi, S., Saltiel, A.R., Kioka, N.
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Journal Title
Journal of Cell Science
Volume: 130
Issue: 20
Pages: 3517-3531
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Conformational Plasticity of JRAB/MICAL-L2 Provides 'Law and Order'2016
Author(s)
A. Sakane, S. Yoshizawa, M. Nishimura, Y. Tsuchiya, N. Matsushita, K. Miyake, K. Horikawa, I. Imoto, C. Mizuguchi, H. Saito, T. Ueno, S. Matsushita, H. Haga, S. Deguchi, K. Mizuguchi, H.
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Journal Title
Molecular Biology of the Cell
Volume: 27
Issue: 20
Pages: 3095-3108
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 細胞が発生する力の評価2016
Author(s)
出口真次
Organizer
名市大・医・Department seminar
Place of Presentation
名古屋市立大学
Year and Date
2016-01-25
Related Report
Invited
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[Presentation] The effect of substrate curvature on myosin-based frictional slip and elongation of focal adhesions2015
Author(s)
Araki, T., Yokoyama, S., Matsui, T.S., Ohishi, T., Kato, K., Deguchi, S.
Organizer
2015 BMES Annual Meeting
Place of Presentation
Tampa Convention Center
Year and Date
2015-10-07
Related Report
Int'l Joint Research
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[Presentation] New cellular traction force microscopy reveals the effect of substrate curvature on focal adhesion dynamics2015
Author(s)
Deguchi, S., Yokoyama, S., Matsui, T.S., Kato, K., Araki, T., Ohishi, T.
Organizer
ATEM'15: International Conference on Advanced Technology in Experimental Mechanics 2015
Place of Presentation
ロジワールホテル豊橋
Year and Date
2015-10-04
Related Report
Int'l Joint Research
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[Presentation] Role of nonmuscle myosin regulatory light chain phosphorylation in contractile force generation2015
Author(s)
Deguchi, S., Yokoyama, S., Matsui, T.S., Kato, K., Araki, T., Ohishi, T., Kuragano, M., Takahashi, M.
Organizer
15th International Congress of Biorheology
Place of Presentation
韓国・高麗大学
Year and Date
2015-05-24
Related Report
Int'l Joint Research / Invited
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[Book] 高度物理刺激と生体応答2017
Author(s)
分担執筆（2.2.7節）：出口真次
Total Pages
198
Publisher
養賢堂
ISBN
9784842505626
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