Development of platelet generation system through waved miRNA regulation
| Project/Area Number |
15H03005
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
ETO KOJI 京都大学, iPS細胞研究所, 教授 (50286986)
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| Co-Investigator(Renkei-kenkyūsha) |
ARAI Fumihito 名古屋大学, 工学研究科(研究院), 教授 (90221051)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | 血小板 / バイオリアクター / ずり応力 / 大量培養 / iPS細胞 / 巨核球 / miRNA / ずり応用 |
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| Outline of Final Research Achievements |
We aimed to establish a novel "megakaryocyte maturation / platelet release system" that enables to generate bona fide platelets with the same function as in vivo with high efficiency. Based on the live bone marrow observation of mouse megakaryocytes protruding their cytoplasm into sinusoid blood vessels and producing platelets under blood flow stimulation, we analyzed flow vectors in those images. The results indicated that turbulence in the blood flow are present adjacent to the cell wall of megakaryocytes from which platelet production event is continuously observed. We then prepared a new model culture tank that demonstrated new mechanisms of how turbulence regulates remodeling of megakaryocytes and promotes platelet production. Using this model, we found conditions that enabled to produce 100 billion platelets by 8L-scale tank. These findings led to creating a new research area of a turbulent flow-controlled thrombopoiesis mechanism.
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Report
(4 results)
Research Products
(83 results)
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[Journal Article] Generating Blood from iPS Cells.2017
Author(s)
Peter Karagiannias, Hiroshi Endo, Koji Eto
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Journal Title
Molecular and Cellular Biology of Platelet Formation: Implications in Health and Disease
Volume: 1
Pages: 399-420
DOI
ISBN
9783319395609, 9783319395623
Related Report
Peer Reviewed
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[Journal Article] IL-1 alpha induces rapid enhanced platelet biogenesis through rupture of Running title: Rupture thrombopoiesis regulated by IL-1alpha murine bone marrow megakaryocytes2015
Author(s)
Satoshi Nishimura, Mika Nagasaki, Shinji Kunishima, Asuka Sakata, Hiroyasu Sakaguchi, Naoya Takayama, Issei Komuro, Joe Italiano Jr., Tomiko Ryu, Tsukasa Ohmori, Takashi Kadowaki, Koji Eto, Ryozo Nagai
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Journal Title
Journal of cell biology
Volume: 未
Issue: 3
Pages: 453-66
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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