Functional and genetical study on protein leakage from glomeruli at the early stage of diabetes
| Project/Area Number |
15H03012
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
矢田 豊隆 川崎医科大学, 医学部, 講師 (00210279)
小笠原 康夫 川崎医療福祉大学, 医療技術学部, 教授 (10152365)
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| Co-Investigator(Renkei-kenkyūsha) |
Kajiya Fumihiko 川崎医療福祉大学, 医療技術学部, 教授 (70029114)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Keywords | 糖尿病 / 糸球体濾過 / スリット膜 / NO / ネフリン / 炎症 / 血管新生 / 貧血 / 遺伝子 / 蛋白尿 / 共焦点顕微鏡 / 蛋白漏出 / 生物・生体工学 / Western Blotting / 微量アルブミン尿 / 早期糖尿病 / RT－PCR法 / ポドシン / CD2AP |
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| Outline of Final Research Achievements |
The purpose of this study is to elucidate the mechanism how C-peptide correct the hyperfiltration and protein leakage from the glomerulus at the early stage of diabetes in a rat. C-peptide did not affect the expression of nephrin, one of the component proteins of slit membrane. The effect of C-peptide towards iNOS, IL-6, CRP, EPO and VEGF was also examined. The only difference we found was that iNOS concentration was decreased by C-peptide administration. The reduction of iNOS may have contributed to correcting the hyperfiltration and protein leakage from the glomerulus. C-peptide, is considered, affects the rodent glomeruli functionally but not structurally.
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Report
(4 results)
Research Products
(11 results)
