Understanding molecular mechanism in human pluripotent stem cell self-renewal and development of synthetic culture system
| Project/Area Number |
15H03022
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Research Collaborator |
Pera Martin F The Jackson Laboratory, Professor
Kahn Michael City of Hope, Department of Molecular Medicine, Professor
Wu Jun UT Southwestern, Department of Molecular Biology, Assistant Professor
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 再生医科学 / 医工学 / ケミカルバイオロジー / 細胞生物学 / 多能性幹細胞 / 化学合成培地 / 培養システム / 自己複製 / 細胞・組織 / 生体材料 / 再生医学 / 幹細胞 / 再生医工学材料 / ヒト多能性幹細胞 / シグナル経路 / 代謝 / 合成培地 |
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| Outline of Final Research Achievements |
Human pluripotent stem cells including ES and iPS cells have ability of infinitely self-renewal and differentiation into all major lineages of cells in the human body. A chemically-defined culture system, free of any biomaterials, is the ideal platform for the quality-controlled, large-scale, cost-effective production of human pluripotent stem cells for use in cell-based therapy and drug discovery. One major objective in developing such a system is to reduce the costs associated with recombinant proteins used as supplements in basal culture media. In this project, we have developed a a defind culture system including growth factor-free culture medium utilizing only three chemical compounds and the least number of recombinant proteins of any other commercially available medium. This culture system may serve as a new benchmark for the development of completely xeno-free, chemically defined, synthetic culture systems.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Nano-on-micro fibrous extracellular matrices for scalable expansion of human ES/iPS cells2017
Author(s)
Li Liu, Ken-ichiro Kamei, Momoko Yoshioka, Minako Nakajima, Junjun Li, Nanae Fujimoto Shiho Terada, Yumie Tokunaga, Yoshie Koyama, Hideki Sato, Koichi Hasegawa, Norio Nakatsuji, Yong Chen
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Journal Title
Biomaterial
Volume: 124
Pages: 47-54
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Growth Factor-free, Chemically-defined Culture System for Maintenance and Derivation of Human Pluripotent Stem Cells2016
Author(s)
Shin-ya Yasuda, Tatsuhiko Ikeda, Noriko Yoshida, Nami Asari, Hosein Shahsavarani, Yuriko Manabe, Atsuko Ueda, Bhavana Nayer, Othmar Korn, Rowland Mosbergen, Christine Wells, Neha Vartak and Kouichi Hasegawa
Organizer
CiRA-ISSCR international symposium 2016
Place of Presentation
京都
Year and Date
2016-03-22
Related Report
Int'l Joint Research
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